Effects of protostane-type triterpenoids on the 5-HT3A receptor-mediated ion current in Xenopus oocytes

Jun Ho Lee; Yoo Jin Lee; Seok Woo Kang; Yangseok Kim; Minkyu Shin; Moochang Hong; Eun Kyoung Seo; Sung Hoon Kim; Seung Yeol Nah; Hyunsu Bae

doi:10.1016/j.brainres.2010.03.041

Effects of protostane-type triterpenoids on the 5-HT₃A receptor-mediated ion current in Xenopus oocytes

Jun Ho Lee, Yoo Jin Lee, Seok Woo Kang, Yangseok Kim, Minkyu Shin, Moochang Hong, Eun Kyoung Seo, Sung Hoon Kim, Seung Yeol Nah, Hyunsu Bae

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT₃A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I_5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT₃A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.

Original language	English
Pages (from-to)	20-27
Number of pages	8
Journal	Brain Research
Volume	1331
DOIs	https://doi.org/10.1016/j.brainres.2010.03.041
State	Published - 17 May 2010

Bibliographical note

Funding Information:
This work was supported by a grant from the Kyung-Hee University Post-Doctoral fellowship in 2009 ( KHU-20090503 )

Keywords

5-HTA receptor
Triterpenoid
Two-electrode voltage clamp

Access to Document

10.1016/j.brainres.2010.03.041

Cite this

@article{fa5bb62c2a0340968dce68848422304e,

title = "Effects of protostane-type triterpenoids on the 5-HT3A receptor-mediated ion current in Xenopus oocytes",

abstract = "Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT3A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT3A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.",

keywords = "5-HTA receptor, Triterpenoid, Two-electrode voltage clamp",

author = "Lee, {Jun Ho} and Lee, {Yoo Jin} and Kang, {Seok Woo} and Yangseok Kim and Minkyu Shin and Moochang Hong and Seo, {Eun Kyoung} and Kim, {Sung Hoon} and Nah, {Seung Yeol} and Hyunsu Bae",

note = "Funding Information: This work was supported by a grant from the Kyung-Hee University Post-Doctoral fellowship in 2009 ( KHU-20090503 ) ",

year = "2010",

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doi = "10.1016/j.brainres.2010.03.041",

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AU - Lee, Jun Ho

AU - Lee, Yoo Jin

AU - Kang, Seok Woo

AU - Kim, Yangseok

AU - Shin, Minkyu

AU - Hong, Moochang

AU - Seo, Eun Kyoung

AU - Kim, Sung Hoon

AU - Nah, Seung Yeol

AU - Bae, Hyunsu

N1 - Funding Information: This work was supported by a grant from the Kyung-Hee University Post-Doctoral fellowship in 2009 ( KHU-20090503 )

PY - 2010/5/17

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N2 - Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT3A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT3A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.

AB - Alisol derivatives are unique protostane-type triterpenoid compounds that are isolated from Alismatis rhizoma, which is a well-known traditional medicine in East Asia. In the present study, we investigated the effects of protostane-type triterpenoids (AA, Alisol A; AB, Alisol B; AB-ac, Alisol B 23-acetate; AC-ac, Alisol C 23-aceteate) on 5-HT-induced currents mediated by the human 5-HT3A receptor expressed in Xenopus laevis oocytes. Co-treatment with triterpenoids regulated the 5-HT-induced inward peak current in a concentration-dependent and reversible manner. In addition, regulation of I5-HT by triterpenoids occurred in a non-competitive manner. Taken together, these results indicate that triterpenoids may regulate the 5-HT3A receptors that are expressed in Xenopus oocytes. Furthermore, this regulation of the ligand-gated ion channel activity by triterpenoids may be one of the pharmacological actions of Alismatis rhizoma.

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