Effects of oxygen free radicals on extracellullar glutamate accumulation in cultured cells

Chang Sik Shin; Seikwan Oh; Ki Wan Oh; Myung Koo Lee; Hack Seang Kim

doi:10.1007/BF02976848

Effects of oxygen free radicals on extracellullar glutamate accumulation in cultured cells

Chang Sik Shin, Seikwan Oh, Ki Wan Oh, Myung Koo Lee, Hack Seang Kim

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Exogenously applied oxygen free radical generating agent, pyrogallol, highly elevated extracellular glutamate accumulation and augmented N-methyl-D-aspartate (NMDA)-induced glutamate accumulation in cerebellar granule neuronal cells, but did not in astrocytes. Superoxide dismutase remarkably decreased the pyrogallol-induced glutamate accumulation, but either NMDA or kainate antagonists did not. In addition, pyrogallol did not affect the NMDA-induced intracellular calcium elevation. Pyrogallol partially blocked glutamate uptake into astrocytes. These results suggest that oxygen free radicals elevate extracellular glutamate accumulation by stimulating the release of glutamate as well as blocking the glutamate uptake.

Original language	English
Pages (from-to)	132-136
Number of pages	5
Journal	Archives of Pharmacal Research
Volume	19
Issue number	2
DOIs	https://doi.org/10.1007/BF02976848
State	Published - 1996

Keywords

Astrocyte
Cerebellar granule neuron
Glutamate accumulation
Intracellular calcium
N-Methyl-D-aspartate
Oxygen free radicals
Pyrogallol

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title = "Effects of oxygen free radicals on extracellullar glutamate accumulation in cultured cells",

abstract = "Exogenously applied oxygen free radical generating agent, pyrogallol, highly elevated extracellular glutamate accumulation and augmented N-methyl-D-aspartate (NMDA)-induced glutamate accumulation in cerebellar granule neuronal cells, but did not in astrocytes. Superoxide dismutase remarkably decreased the pyrogallol-induced glutamate accumulation, but either NMDA or kainate antagonists did not. In addition, pyrogallol did not affect the NMDA-induced intracellular calcium elevation. Pyrogallol partially blocked glutamate uptake into astrocytes. These results suggest that oxygen free radicals elevate extracellular glutamate accumulation by stimulating the release of glutamate as well as blocking the glutamate uptake.",

keywords = "Astrocyte, Cerebellar granule neuron, Glutamate accumulation, Intracellular calcium, N-Methyl-D-aspartate, Oxygen free radicals, Pyrogallol",

author = "Shin, {Chang Sik} and Seikwan Oh and Oh, {Ki Wan} and Lee, {Myung Koo} and Kim, {Hack Seang}",

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T1 - Effects of oxygen free radicals on extracellullar glutamate accumulation in cultured cells

AU - Shin, Chang Sik

AU - Oh, Seikwan

AU - Oh, Ki Wan

AU - Lee, Myung Koo

AU - Kim, Hack Seang

PY - 1996

Y1 - 1996

N2 - Exogenously applied oxygen free radical generating agent, pyrogallol, highly elevated extracellular glutamate accumulation and augmented N-methyl-D-aspartate (NMDA)-induced glutamate accumulation in cerebellar granule neuronal cells, but did not in astrocytes. Superoxide dismutase remarkably decreased the pyrogallol-induced glutamate accumulation, but either NMDA or kainate antagonists did not. In addition, pyrogallol did not affect the NMDA-induced intracellular calcium elevation. Pyrogallol partially blocked glutamate uptake into astrocytes. These results suggest that oxygen free radicals elevate extracellular glutamate accumulation by stimulating the release of glutamate as well as blocking the glutamate uptake.

AB - Exogenously applied oxygen free radical generating agent, pyrogallol, highly elevated extracellular glutamate accumulation and augmented N-methyl-D-aspartate (NMDA)-induced glutamate accumulation in cerebellar granule neuronal cells, but did not in astrocytes. Superoxide dismutase remarkably decreased the pyrogallol-induced glutamate accumulation, but either NMDA or kainate antagonists did not. In addition, pyrogallol did not affect the NMDA-induced intracellular calcium elevation. Pyrogallol partially blocked glutamate uptake into astrocytes. These results suggest that oxygen free radicals elevate extracellular glutamate accumulation by stimulating the release of glutamate as well as blocking the glutamate uptake.

KW - Astrocyte

KW - Cerebellar granule neuron

KW - Glutamate accumulation

KW - Intracellular calcium

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JO - Archives of Pharmacal Research

JF - Archives of Pharmacal Research

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Effects of oxygen free radicals on extracellullar glutamate accumulation in cultured cells

Abstract

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