Effects of isorhamnetin on adipocyte mitochondrial biogenesis and ampk activation

Mak Soon Lee, Yangha Kim

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Isorhamnetin (ISOR), 3-O-methylquercetin, is a naturally occurring flavonoid in many plants. It is a metabolite derived from quercetin and is known to exert beneficial effects on the prevention of obesity. However, the molecular mechanism of action involved in ISOR-mediated mitochondrial biogenesis, and AMP-activated protein kinase (AMPK) activation in 3T3-L1 cells remains unclear. The aim of this study was to determine whether ISOR affected mitochondrial biogenesis and AMPK activation, during 3T3-L1 adipocyte differentiation. Intracellular lipid and triglyceride accumulation, and glycerol-3-phosphate dehydrogenase (GPDH) activity decreased in ISOR-treated cells. The mRNA levels of adipogenic genes, such as the proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2), were inhibited by ISOR. In contrast, mRNA levels of mitochondrial genes, such as peroxisome proliferator-activated reporter gamma coactivator-1α (PGC-1α), nuclear respiratory factor (NRF)-1, transcription factor A (Tfam), and carnitine palmitoyl transferase-1α (CPT-1α), were all stimulated by ISOR treatment. Mitochondria DNA (mtDNA) copy number and AMPK activity were also stimulated by ISOR. The results suggested that the mitochondrial biogenic effect of ISOR in adipocytes might have been associated with stimulation of mitochondrial gene expression, mtDNA replication, and AMPK activation.

Original languageEnglish
Article number1853
JournalMolecules
Volume23
Issue number8
DOIs
StatePublished - 2018

Bibliographical note

Funding Information:
Funding: This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science & ICT (numbers 2012M3A9C4048761 and 2016R1A2B4011021).

Publisher Copyright:
© 2018 by the authors.

Keywords

  • 3T3-L1 adipocytes
  • AMPK
  • Adipogenesis
  • Isorhamnetin
  • Mitochondrial biogenesis

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