Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants

So Hee Kim; Soon Sun Kim; Oran Kwon; Kyung Hee Sohn; Seung Jun Kwack; Yo Woo Choi; Soon Young Han; Myung Koo Lee; Kui Lea Park

doi:10.1080/15287390252808118

Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants

So Hee Kim, Soon Sun Kim, Oran Kwon, Kyung Hee Sohn, Seung Jun Kwack, Yo Woo Choi, Soon Young Han, Myung Koo Lee, Kui Lea Park

Department of Nutritional Science & Food Management

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

This present study was undertaken to examine the effects of DBP and its metabolite mono-n-butyl phthalate (MBuP) on cytotoxicity and differentiation in cultured rat embryonic limb bud cells. When limb bud cells extracted from rats on gestation d 12.5 were treated with DBP or MBuP for 96 h, induction of cytotoxicity and inhibition of cell differentiation were observed in a concentration-dependent manner. However, MBuP elicited a toxic effect at higher concentrations than DBP. The IC50 values of DBP for cytotoxicity (measured by neutral red uptake) and cell differentiation (measured by alcian blue staining) were 25.54 μg/ml (91.75 μM) and 21.21 μg/ml (76.20 μM), respectively. The IC50 values of MBuP for cytotoxicity and cell differentiation were 307.24 μg/ml (1.38 mM) and 142.61 μg/ml (0.64 mM), respectively. In order to determine whether free radicals are related to induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells, DBP was coadministered with several antioxidants, including catalase and vitamin E acetate to limb bud cells. Cotreatment with catalase and vitamin E acetate decreased induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells. However, these compounds did not show any protective effect against MBuP. Results indicate that DBP and MBuP induced developmental toxicity in rat embryonic limb bud cells and suggest that this effect of DBP might be exerted through oxidative stress.

Original language	English
Pages (from-to)	461-472
Number of pages	12
Journal	Journal of Toxicology and Environmental Health - Part A
Volume	65
Issue number	5-6
DOIs	https://doi.org/10.1080/15287390252808118
State	Published - 2002

Bibliographical note

Funding Information:
This study was supported by a reasrfeucdfhrmn tohKorea eFoo d and Drug Administration (2000). AddrescsrosropedenncetoKuLeiPaaReprroukctve,ida Dnedpmelentoval TxicoloogyDiv ision, National Institute of Toxicological Research, KoreaFoo d and Drug Administration, Seoul 122-704, Ko.rEea-i:plam [email protected].

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10.1080/15287390252808118

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Kim, S. H., Kim, S. S., Kwon, O., Sohn, K. H., Kwack, S. J., Choi, Y. W., Han, S. Y., Lee, M. K., & Park, K. L. (2002). Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants. Journal of Toxicology and Environmental Health - Part A, 65(5-6), 461-472. https://doi.org/10.1080/15287390252808118

@article{bc125b253e3748dc88d6f34fad6de053,

title = "Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants",

abstract = "This present study was undertaken to examine the effects of DBP and its metabolite mono-n-butyl phthalate (MBuP) on cytotoxicity and differentiation in cultured rat embryonic limb bud cells. When limb bud cells extracted from rats on gestation d 12.5 were treated with DBP or MBuP for 96 h, induction of cytotoxicity and inhibition of cell differentiation were observed in a concentration-dependent manner. However, MBuP elicited a toxic effect at higher concentrations than DBP. The IC50 values of DBP for cytotoxicity (measured by neutral red uptake) and cell differentiation (measured by alcian blue staining) were 25.54 μg/ml (91.75 μM) and 21.21 μg/ml (76.20 μM), respectively. The IC50 values of MBuP for cytotoxicity and cell differentiation were 307.24 μg/ml (1.38 mM) and 142.61 μg/ml (0.64 mM), respectively. In order to determine whether free radicals are related to induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells, DBP was coadministered with several antioxidants, including catalase and vitamin E acetate to limb bud cells. Cotreatment with catalase and vitamin E acetate decreased induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells. However, these compounds did not show any protective effect against MBuP. Results indicate that DBP and MBuP induced developmental toxicity in rat embryonic limb bud cells and suggest that this effect of DBP might be exerted through oxidative stress.",

author = "Kim, {So Hee} and Kim, {Soon Sun} and Oran Kwon and Sohn, {Kyung Hee} and Kwack, {Seung Jun} and Choi, {Yo Woo} and Han, {Soon Young} and Lee, {Myung Koo} and Park, {Kui Lea}",

note = "Funding Information: This study was supported by a reasrfeucdfhrmn tohKorea eFoo d and Drug Administration (2000). AddrescsrosropedenncetoKuLeiPaaReprroukctve,ida Dnedpmelentoval TxicoloogyDiv ision, National Institute of Toxicological Research, KoreaFoo d and Drug Administration, Seoul 122-704, Ko.rEea-i:plam [email protected].",

year = "2002",

doi = "10.1080/15287390252808118",

language = "English",

volume = "65",

pages = "461--472",

journal = "Journal of Toxicology and Environmental Health - Part A",

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Kim, SH, Kim, SS, Kwon, O, Sohn, KH, Kwack, SJ, Choi, YW, Han, SY, Lee, MK & Park, KL 2002, 'Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants', Journal of Toxicology and Environmental Health - Part A, vol. 65, no. 5-6, pp. 461-472. https://doi.org/10.1080/15287390252808118

Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants. / Kim, So Hee; Kim, Soon Sun; Kwon, Oran et al.
In: Journal of Toxicology and Environmental Health - Part A, Vol. 65, No. 5-6, 2002, p. 461-472.

Research output: Contribution to journal › Article › peer-review

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T1 - Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants

AU - Kim, So Hee

AU - Kim, Soon Sun

AU - Kwon, Oran

AU - Sohn, Kyung Hee

AU - Kwack, Seung Jun

AU - Choi, Yo Woo

AU - Han, Soon Young

AU - Lee, Myung Koo

AU - Park, Kui Lea

N1 - Funding Information: This study was supported by a reasrfeucdfhrmn tohKorea eFoo d and Drug Administration (2000). AddrescsrosropedenncetoKuLeiPaaReprroukctve,ida Dnedpmelentoval TxicoloogyDiv ision, National Institute of Toxicological Research, KoreaFoo d and Drug Administration, Seoul 122-704, Ko.rEea-i:plam [email protected].

PY - 2002

Y1 - 2002

N2 - This present study was undertaken to examine the effects of DBP and its metabolite mono-n-butyl phthalate (MBuP) on cytotoxicity and differentiation in cultured rat embryonic limb bud cells. When limb bud cells extracted from rats on gestation d 12.5 were treated with DBP or MBuP for 96 h, induction of cytotoxicity and inhibition of cell differentiation were observed in a concentration-dependent manner. However, MBuP elicited a toxic effect at higher concentrations than DBP. The IC50 values of DBP for cytotoxicity (measured by neutral red uptake) and cell differentiation (measured by alcian blue staining) were 25.54 μg/ml (91.75 μM) and 21.21 μg/ml (76.20 μM), respectively. The IC50 values of MBuP for cytotoxicity and cell differentiation were 307.24 μg/ml (1.38 mM) and 142.61 μg/ml (0.64 mM), respectively. In order to determine whether free radicals are related to induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells, DBP was coadministered with several antioxidants, including catalase and vitamin E acetate to limb bud cells. Cotreatment with catalase and vitamin E acetate decreased induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells. However, these compounds did not show any protective effect against MBuP. Results indicate that DBP and MBuP induced developmental toxicity in rat embryonic limb bud cells and suggest that this effect of DBP might be exerted through oxidative stress.

AB - This present study was undertaken to examine the effects of DBP and its metabolite mono-n-butyl phthalate (MBuP) on cytotoxicity and differentiation in cultured rat embryonic limb bud cells. When limb bud cells extracted from rats on gestation d 12.5 were treated with DBP or MBuP for 96 h, induction of cytotoxicity and inhibition of cell differentiation were observed in a concentration-dependent manner. However, MBuP elicited a toxic effect at higher concentrations than DBP. The IC50 values of DBP for cytotoxicity (measured by neutral red uptake) and cell differentiation (measured by alcian blue staining) were 25.54 μg/ml (91.75 μM) and 21.21 μg/ml (76.20 μM), respectively. The IC50 values of MBuP for cytotoxicity and cell differentiation were 307.24 μg/ml (1.38 mM) and 142.61 μg/ml (0.64 mM), respectively. In order to determine whether free radicals are related to induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells, DBP was coadministered with several antioxidants, including catalase and vitamin E acetate to limb bud cells. Cotreatment with catalase and vitamin E acetate decreased induction of cytotoxicity and inhibition of differentiation by DBP in limb bud cells. However, these compounds did not show any protective effect against MBuP. Results indicate that DBP and MBuP induced developmental toxicity in rat embryonic limb bud cells and suggest that this effect of DBP might be exerted through oxidative stress.

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Effects of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants

Abstract

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