Effects of carbon source, C/N ratio, nitrate, temperature, and pH on N 2 O emission and functional denitrifying genes during heterotrophic denitrification

Yun Yeong Lee, Hyungjoo Choi, Kyung Suk Cho

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The effects of operational parameters such as carbon source, C/N ratio, initial nitrate concentration, temperature, and pH value on heterotrophic denitrification and functional denitrifying genes were evaluated. When methanol was used as the sole carbon source, complete denitrification was performed in a short time without nitrous oxide (N 2 O) emission. Complete denitrification was performed at high C/N ratios (5.14 and 12.85) and low initial nitrate concentrations (75.9 and 151.6 mg N L −1 ). The denitrification rate was not temperature-sensitive in the range of 25–35 °C, but tended to decrease at a low pH of 5–6. The relationships between N 2 O emission and functional genes under various operational conditions were investigated by Pearson correlation and association network analyses. The C/N ratio was a key factor for N 2 O emission during the heterotrophic denitrification process. This information on the denitrification performance and its association with functional gene dynamics under various operational conditions is useful for N 2 O mitigation strategies for wastewater treatment processes.

Original languageEnglish
Pages (from-to)16-29
Number of pages14
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume54
Issue number1
DOIs
StatePublished - 2 Jan 2019

Keywords

  • C/N ratio
  • functional genes
  • Heterotrophic denitrification
  • nitrous oxide
  • operational parameters

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