Effects of a tetramethoxyhydroxyflavone on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells

Doyoung Yoon, Minchul Cho, Jung Hee Kim, Eun Jin Kim, Jeong Woo Kang, Eun Hee Seo, Jung Hyun Shim, Soo Hyun Kim, Hee Gu Lee, Goo Taeg Oh, Jin Tae Hong, Joo Won Park, Jong Wan Kim

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The inhibitory effects of 5,6,3′,5′-tetramethoxy 7,4′-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1β or TNF-α induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin E2 in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1β and thus proliferates), revealing that p7F inhibited IL-1β-induced proliferation of D10S Th2 cells in a dose-response manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IκB degradation and NF-κB activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an anti-inflammatory agent for inhibiting inflammatory responses.

Original languageEnglish
Pages (from-to)686-694
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume18
Issue number4
StatePublished - 28 Apr 2008

Keywords

  • Anti-inflammatory agent
  • Flavoric
  • Inflammatory mediators
  • Proinflammatory cytokines
  • Synovial fibroblasts

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