Abstract
The inhibitory effects of 5,6,3′,5′-tetramethoxy 7,4′-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1β or TNF-α induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin E2 in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1β and thus proliferates), revealing that p7F inhibited IL-1β-induced proliferation of D10S Th2 cells in a dose-response manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IκB degradation and NF-κB activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an anti-inflammatory agent for inhibiting inflammatory responses.
Original language | English |
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Pages (from-to) | 686-694 |
Number of pages | 9 |
Journal | Journal of Microbiology and Biotechnology |
Volume | 18 |
Issue number | 4 |
State | Published - 28 Apr 2008 |
Keywords
- Anti-inflammatory agent
- Flavoric
- Inflammatory mediators
- Proinflammatory cytokines
- Synovial fibroblasts