TY - JOUR
T1 - Effective screening for the anti-hypertensive of selected herbs used in the traditional Korean medicines
AU - Kim, Ji Yeon
AU - Kim, Doram
AU - Kwon, Oran
N1 - Funding Information:
This study was supported by the Bio-Synergy Research Project of the Ministry of Science, ICT and Future Planning (NRF Project No. 2013M3A9C4078153).
Publisher Copyright:
© 2016, The Korean Society for Applied Biological Chemistry.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - In this study, Korean medicinal herb extracts were investigated for their in vitro effect on anti-hypertensive activities, including nitric oxide (NO) production in the human umbilical vein endothelial cell line EA.hy529 and angiotensin-converting enzyme (ACE) activity. Traditional remedies related to the treatment of hypertension were identified using DongUiBoGam, the most comprehensive source for Korean traditional pharmacopoeia. Herbal remedies were selected by translating the symptom terminology into westernized medicinal terms using the Korean classification of disease in Oriental medicine and Korea Traditional Knowledge Portal. Seven types of herb extracts were selected and screened for in vitro anti-hypertensive activities. Among the extracts, those of Evodiae fructus, Bambusae caulis in Taeniam, Sophorae fructus, Melonis calyx, and Schizonepeta rhizome produced significant increases in NO production, although their ability to inhibit ACE were weaker. Using Fourier transform ion cyclotron resonance mass spectrometry, the major flavonoids exhibiting anti-hypertensive activities were identified: quercetin, apigenin, and luteolin. Our results indicate that these medicinal herbs are potent natural anti-hypertensive agents that can be developed for clinical therapies. In addition, our systematic approach to identifying candidate herbal remedies in the traditional Korean pharmacopoeia can be used to search for additional traditional remedies, such as for hypoglycemia and obesity.
AB - In this study, Korean medicinal herb extracts were investigated for their in vitro effect on anti-hypertensive activities, including nitric oxide (NO) production in the human umbilical vein endothelial cell line EA.hy529 and angiotensin-converting enzyme (ACE) activity. Traditional remedies related to the treatment of hypertension were identified using DongUiBoGam, the most comprehensive source for Korean traditional pharmacopoeia. Herbal remedies were selected by translating the symptom terminology into westernized medicinal terms using the Korean classification of disease in Oriental medicine and Korea Traditional Knowledge Portal. Seven types of herb extracts were selected and screened for in vitro anti-hypertensive activities. Among the extracts, those of Evodiae fructus, Bambusae caulis in Taeniam, Sophorae fructus, Melonis calyx, and Schizonepeta rhizome produced significant increases in NO production, although their ability to inhibit ACE were weaker. Using Fourier transform ion cyclotron resonance mass spectrometry, the major flavonoids exhibiting anti-hypertensive activities were identified: quercetin, apigenin, and luteolin. Our results indicate that these medicinal herbs are potent natural anti-hypertensive agents that can be developed for clinical therapies. In addition, our systematic approach to identifying candidate herbal remedies in the traditional Korean pharmacopoeia can be used to search for additional traditional remedies, such as for hypoglycemia and obesity.
KW - Angiotensin-converting enzyme inhibition
KW - Anti-hypertensive activity
KW - DongUiBoGam
KW - Medicinal herb
KW - Nitric oxide
KW - Traditional Korean pharmacopoeia
UR - http://www.scopus.com/inward/record.url?scp=84976484008&partnerID=8YFLogxK
U2 - 10.1007/s13765-016-0190-7
DO - 10.1007/s13765-016-0190-7
M3 - Article
AN - SCOPUS:84976484008
SN - 2468-0834
VL - 59
SP - 525
EP - 532
JO - Applied Biological Chemistry
JF - Applied Biological Chemistry
IS - 4
ER -