Effective inactivated influenza vaccine for the elderly using a single-stranded RNA-based adjuvant

Yoo Jin Bang, So Hee Hong, Hyo Jung Park, Hye Won Kwak, Yu Sun Lee, Jae Yong Kim, Hyeong Jun Park, Seo Hyeon Bae, Hye Jung Kim, Yun Hee Kim, Hae Li Ko, Sang In Park, Hun Kim, Gyeongjoo Park, Man Seong Park, Jun Chang, Jae Hwan Nam

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There is an unmet need for new influenza vaccine strategies that compensate for impaired vaccine responses in elderly individuals. Here, we evaluated the effectiveness of a single-stranded RNA (ssRNA) as an adjuvant to enhance the efficacy of inactivated influenza vaccine (IIV) in mouse models. Immunization with the ssRNA along with IIV reduced viral titers as well as pathological and inflammatory scores in the lungs after influenza challenge in aged mice. ssRNA induced balanced Th1/Th2 responses with an increase in IgA titers. Moreover, the ssRNA adjuvant markedly increased the frequency of influenza HA-specific T cells and IFN-γ production along with the expression of genes related to innate and adaptive immune systems that could overcome immunosenescence in aged mice. Our findings indicate that ssRNA is an efficient vaccine adjuvant that boosts cellular and humoral immunity in aged mice, demonstrating its potential as a novel adjuvant for currently available influenza virus vaccines for elderly individuals.

Original languageEnglish
Article number11981
JournalScientific Reports
Issue number1
StatePublished - Dec 2021

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© 2021, The Author(s).


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