Effect of trichloroethylene and tetrachloroethylene on methane oxidation and community structure of methanotrophic consortium

Sun Ah Choi, Eun Hee Lee, Kyung Suk Cho

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8 Scopus citations

Abstract

The methane oxidation rate and community structure of a methanotrophic consortium were analyzed to determine the effects of trichloroethylene (TCE) and tetrachloroethylene (PCE) on methane oxidation. The maximum methane oxidation rate(Vmax) of the consortium was 326.8 μmol·g-dry biomass-1·h-1, and it had a half-saturation constant(Km) of 143.8 μM. The addition of TCE or PCE resulted in decreased methane oxidation rates, which were decreased from 101.73 to 5.47-24.64 μmol·g-dry biomass-1·h-1 with an increase in the TCE-to-methane ratio, and to 61.95-67.43 μmol·g-dry biomass-1·h-1 with an increase in the PCE-to-methane ratio. TCE and PCE were non-competitive inhibitors for methane oxidation, and their inhibition constants(Ki) were 33.4 and 132.0 μM, respectively. When the methanotrophic community was analyzed based on pmoA using quantitative real-time PCR (qRT-PCR), the pmoA gene copy numbers were shown to decrease from 7.3 ± 0.7 × 108 to 2.1-5.0 × 107 pmoA gene copy number · g-dry biomass-1 with an increase in the TCE-to-methane ratio and to 2.5-7.0 × 10 7 pmoA gene copy number · g-dry biomass-1 with an increase in the PCE-to-methane ratio. Community analysis by microarray demonstrated that Methylocystis (type II methanotrophs) were the most abundant in the methanotrophic community composition in the presence of TCE. These results suggest that toxic effects caused by TCE and PCE change not only methane oxidation rates but also the community structure of the methanotrophic consortium.

Original languageEnglish
Pages (from-to)1723-1731
Number of pages9
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume48
Issue number13
DOIs
StatePublished - 10 Nov 2013

Keywords

  • community structure
  • methane
  • Methanotrophic bacteria
  • tetrachloroethylene (PCE)
  • trichloroethylene (TCE)

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