To examine alterations in LHRH and LH gene expression in response to testosterone (T), dihydrotestosterone (DHT) and estradiol (E), LHRH mRNA level in the hypothalamus, and α and LHβ subunit mRNA levels in the anterior pituitary were simultaneously determined. T and other steroids were administered s.c. to castrated (two weeks) rats, and LHRH, α and LHβ mRNA levels were determined by RNA-blot hybridization with appropriate probes. All of the three steroids, T, DHT and E efficiently suppressed α and LHβ mRNA levels in the anterior pituitary, which were accompanied by parallel change in serum LH levels. However, DHT was only effective in decreasing LHRH mRNA level, whereas neither T nor E altered LHRH mRNA levels. The present study indicates that T and its metabolites differentially regulate LHRH and LH gene expression.
|Number of pages||6|
|State||Published - 1994|