Effect of PVA-encapsulation on hydrogen production and bacterial community structure

Jeonghee Yun, Tae Gwan Kim, Kyung Suk Cho

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this study, the performances of PVA-encapsulation and non-encapsulation in a fed-batch bioreactor system were compared for biohydrogen production. Hydrogen production in the PVA-encapsulation bioreactor was not significantly different in comparison to the non-encapsulation bioreactor. However, the hydrogen gas in the encapsulation bioreactor could be stably produced when it was exposed to environmental difficulties such as pH impact by the accumulation of organic acids as fermentative metabolic products. Bacterial communities by DGGE analysis were differently shifted between the PVA-encapsulation and nonencapsulation bioreactors from the initial sludge. The community of hydrogen producing bacteria was stable during the experimental period in the PVA-encapsulation bioreactor compared to the non-encapsulation method. The absolute quantitation of the DNA copy number by a high-throughput droplet digital PCR system for six genera contributed to hydrogen production showing that the numbers of dominant bacteria existed at similar levels in the two bioreactors regardless of encapsulation. In both of two bioreactors, not only Clostridium and Enterobacter, which are known as anaerobic hydrogen producing bacteria, but also Firmicutes, Ruminococcus and Escherichia existed with 1 × 105 -1 &times 106 copy numbers of ml-samples exhibiting rapid growth during the initial operation period.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalKorean Journal of Microbiology and Biotechnology
Volume42
Issue number1
DOIs
StatePublished - Mar 2014

Keywords

  • Bacterial community
  • Encapsulation
  • Hydrogen production
  • Polyvinyl alcohol (PVA)

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