Effect of necrosis on the miRNA-mRNA regulatory network in CRT-MG human astroglioma cells

So Hee Ahn, Jung Hyuck Ahn, Dong Ryeol Ryu, Jisoo Lee, Min Sun Cho, Youn Hee Choi

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Purpose Glioblastoma multiforme (GBM) is the most common adult primary intracranial tumor. The remarkable features of GBM include central necrosis. MicroRNAs (miRNAs) have been considered as diagnostic/prognostic biomarkers for many cancers, including glioblastoma. However, the effect of necrosis on the miRNA expression profile and predicted miRNA-mRNA regulatory information remain unclear. The purpose of this study is to examine the effect of necrotic cells on the modulation of miRNA and mRNA expression profiles and miRNA-mRNA network in CRT-MG cells. Materials and Methods We used human astroglioma cells, CRT-MG, treated with necrotic CRT-MG cells to examine the effect of necrosis on the modulation of miRNA and mRNA by next-generation sequencing. For preparation of necrotic cells, CRT-MG cells were frozen and thawed through cycle of liquid nitrogen-water bath. The putative miRNA-mRNA regulatory relationship was inferred through target information, using miRDB. Results The necrotic cells induced dysregulation of 106 miRNAs and 887 mRNAs. Among them, 11 miRNAs that had a negative correlation value of p < 0.05 by the hypergeometric test were screened, and their target mRNAs were analyzed by Gene Ontology enrichment analysis. Using the Kyoto Encyclopedia of Genes and Genomes database, we also found several necrotic cell treatment-activated pathways that were modulated by relevant gene targets of differentially expressed miRNAs. Conclusion Our result demonstrated that dysregulation of miRNA and mRNA expression profiles occurs when GBM cells are exposed to necrotic cells, suggesting that several miRNAs may have the potential to be used as biomarkers for predicting GBM progression and pathogenesis.

Original languageEnglish
Pages (from-to)382-397
Number of pages16
JournalCancer Research and Treatment
Issue number2
StatePublished - 1 Apr 2018

Bibliographical note

Funding Information:
This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) Grant 2010-0027945 and by NRF-2016R1A2B4016376.

Publisher Copyright:
© 2018 by the Korean Cancer Association.


  • Glioblastoma
  • MicroRNA
  • Necrosis


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