Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

Sun Young Ju; Kyung Ah Cho; Su Jin Cho; Yun Jae Jung; So Youn Woo; Ju Young Seoh; Ho Seong Han; Kyung Ha Ryu

doi:10.1016/j.cellbi.2008.10.002

Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

Sun Young Ju
, Kyung Ah Cho
, Su Jin Cho
, Yun Jae Jung
, So Youn Woo
, Ju Young Seoh
, Ho Seong Han
, Kyung Ha Ryu

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl₄-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl₄ damaged liver.

Original language	English
Pages (from-to)	31-35
Number of pages	5
Journal	Cell Biology International
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.cellbi.2008.10.002
State	Published - Jan 2009

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Research Foundation (KRF-2003-041-E00136).

Keywords

Bone marrow cells
Cell migration
Hypoxia
SDF-1

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10.1016/j.cellbi.2008.10.002

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title = "Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver",

abstract = "Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20\% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl4-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl4 damaged liver.",

keywords = "Bone marrow cells, Cell migration, Hypoxia, SDF-1",

author = "Ju, \{Sun Young\} and Cho, \{Kyung Ah\} and Cho, \{Su Jin\} and Jung, \{Yun Jae\} and Woo, \{So Youn\} and Seoh, \{Ju Young\} and Han, \{Ho Seong\} and Ryu, \{Kyung Ha\}",

note = "Funding Information: This study was supported by a grant from the Korea Research Foundation (KRF-2003-041-E00136).",

year = "2009",

month = jan,

doi = "10.1016/j.cellbi.2008.10.002",

language = "English",

volume = "33",

pages = "31--35",

journal = "Cell Biology International",

issn = "1065-6995",

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T1 - Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

AU - Ju, Sun Young

AU - Cho, Kyung Ah

AU - Cho, Su Jin

AU - Jung, Yun Jae

AU - Woo, So Youn

AU - Seoh, Ju Young

AU - Han, Ho Seong

AU - Ryu, Kyung Ha

N1 - Funding Information: This study was supported by a grant from the Korea Research Foundation (KRF-2003-041-E00136).

PY - 2009/1

Y1 - 2009/1

N2 - Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl4-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl4 damaged liver.

AB - Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl4-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl4 damaged liver.

KW - Bone marrow cells

KW - Cell migration

KW - Hypoxia

KW - SDF-1

UR - https://www.scopus.com/pages/publications/57649155507

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DO - 10.1016/j.cellbi.2008.10.002

M3 - Article

C2 - 18992833

AN - SCOPUS:57649155507

SN - 1065-6995

VL - 33

SP - 31

EP - 35

JO - Cell Biology International

JF - Cell Biology International

IS - 1

ER -

Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

Abstract

Bibliographical note

Keywords

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