Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

Sun Young Ju; Kyung Ah Cho; Su Jin Cho; Yun Jae Jung; So Youn Woo; Ju Young Seoh; Ho Seong Han; Kyung Ha Ryu

doi:10.1016/j.cellbi.2008.10.002

Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

Sun Young Ju, Kyung Ah Cho, Su Jin Cho, Yun Jae Jung, So Youn Woo, Ju Young Seoh, Ho Seong Han, Kyung Ha Ryu

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl₄-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl₄ damaged liver.

Original language	English
Pages (from-to)	31-35
Number of pages	5
Journal	Cell Biology International
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/j.cellbi.2008.10.002
State	Published - Jan 2009

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea Research Foundation (KRF-2003-041-E00136).

Keywords

Bone marrow cells
Cell migration
Hypoxia
SDF-1

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10.1016/j.cellbi.2008.10.002

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title = "Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver",

abstract = "Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl4-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl4 damaged liver.",

keywords = "Bone marrow cells, Cell migration, Hypoxia, SDF-1",

author = "Ju, {Sun Young} and Cho, {Kyung Ah} and Cho, {Su Jin} and Jung, {Yun Jae} and Woo, {So Youn} and Seoh, {Ju Young} and Han, {Ho Seong} and Ryu, {Kyung Ha}",

note = "Funding Information: This study was supported by a grant from the Korea Research Foundation (KRF-2003-041-E00136).",

year = "2009",

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doi = "10.1016/j.cellbi.2008.10.002",

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volume = "33",

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AU - Ju, Sun Young

AU - Cho, Kyung Ah

AU - Cho, Su Jin

AU - Jung, Yun Jae

AU - Woo, So Youn

AU - Seoh, Ju Young

AU - Han, Ho Seong

AU - Ryu, Kyung Ha

N1 - Funding Information: This study was supported by a grant from the Korea Research Foundation (KRF-2003-041-E00136).

PY - 2009/1

Y1 - 2009/1

N2 - Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl4-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl4 damaged liver.

AB - Restricted numbers and poor regenerative properties limit the use of adult stem cells. We tested the effect of hypoxic treatment as a method by which to increase cell migration. Bone marrow cells (BMCs) were cultured under oxygen saturations of 0.1, 3, and 20% for 24 h. After hypoxic treatment, BMCs of apoptotic fraction were decreased. The expression of CXCR4 was noticeably increased in the hypoxia-treated BMCs and their migration in response to SDF-1α was enhanced compared with cells cultured under normoxic condition. Hypoxic BMCs had a higher degree of engraftment to the CCl4-injured liver than the normoxic cells. Hypoxic treatment of BMCs may have merits in decreasing apoptosis of those cells as well as in enhancing cellular migration to SDF-1α, the chemokine which binds to BMCs expressed CXCR4 and to the injured tissue, such as CCl4 damaged liver.

KW - Bone marrow cells

KW - Cell migration

KW - Hypoxia

KW - SDF-1

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JO - Cell Biology International

JF - Cell Biology International

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Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver

Abstract

Bibliographical note

Keywords

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