Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

Dong Mi Shin; Kyeong Mi Choi; Youn Sun Lee; Wonkyun Kim; Kyong Oh Shin; Seikwan Oh; Jae Chul Jung; Mi Kyeong Lee; Yong Moon Lee; Jin Tae Hong; Yeo Pyo Yun; Hwan Soo Yoo

doi:10.1007/s12272-013-0251-y

Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

Dong Mi Shin
, Kyeong Mi Choi
, Youn Sun Lee
, Wonkyun Kim
, Kyong Oh Shin
, Seikwan Oh
, Jae Chul Jung
, Mi Kyeong Lee
, Yong Moon Lee
, Jin Tae Hong
, Yeo Pyo Yun
, Hwan Soo Yoo

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1- methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.

Original language	English
Pages (from-to)	803-812
Number of pages	10
Journal	Archives of Pharmacal Research
Volume	37
Issue number	6
DOIs	https://doi.org/10.1007/s12272-013-0251-y
State	Published - Jun 2014

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013320), the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (MRC, 2010-0029480), the Ministry of Trade, Industry & Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-In-dudtry Convergence (BAIO), and the ‘‘Leaders INdustry-university Cooperation’’ Project funded by the Ministry of Education.

Keywords

3T3-L1 cells
Adipocyte differentiation
Dodeca-2(E),4(E)-dienoic acid isobutylamide
Echinacea purpurea
Insulin

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title = "Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells",

abstract = "Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1- methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.",

keywords = "3T3-L1 cells, Adipocyte differentiation, Dodeca-2(E),4(E)-dienoic acid isobutylamide, Echinacea purpurea, Insulin",

author = "Shin, \{Dong Mi\} and Choi, \{Kyeong Mi\} and Lee, \{Youn Sun\} and Wonkyun Kim and Shin, \{Kyong Oh\} and Seikwan Oh and Jung, \{Jae Chul\} and Lee, \{Mi Kyeong\} and Lee, \{Yong Moon\} and Hong, \{Jin Tae\} and Yun, \{Yeo Pyo\} and Yoo, \{Hwan Soo\}",

note = "Funding Information: Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013320), the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (MRC, 2010-0029480), the Ministry of Trade, Industry \& Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-In-dudtry Convergence (BAIO), and the {\textquoteleft}{\textquoteleft}Leaders INdustry-university Cooperation{\textquoteright}{\textquoteright} Project funded by the Ministry of Education.",

year = "2014",

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doi = "10.1007/s12272-013-0251-y",

language = "English",

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journal = "Archives of Pharmacal Research",

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T1 - Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

AU - Shin, Dong Mi

AU - Choi, Kyeong Mi

AU - Lee, Youn Sun

AU - Kim, Wonkyun

AU - Shin, Kyong Oh

AU - Oh, Seikwan

AU - Jung, Jae Chul

AU - Lee, Mi Kyeong

AU - Lee, Yong Moon

AU - Hong, Jin Tae

AU - Yun, Yeo Pyo

AU - Yoo, Hwan Soo

N1 - Funding Information: Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013320), the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (MRC, 2010-0029480), the Ministry of Trade, Industry & Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-In-dudtry Convergence (BAIO), and the ‘‘Leaders INdustry-university Cooperation’’ Project funded by the Ministry of Education.

PY - 2014/6

Y1 - 2014/6

N2 - Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1- methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.

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KW - 3T3-L1 cells

KW - Adipocyte differentiation

KW - Dodeca-2(E),4(E)-dienoic acid isobutylamide

KW - Echinacea purpurea

KW - Insulin

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JF - Archives of Pharmacal Research

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ER -

Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

Abstract

Bibliographical note

Keywords

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