Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

Dong Mi Shin; Kyeong Mi Choi; Youn Sun Lee; Wonkyun Kim; Kyong Oh Shin; Seikwan Oh; Jae Chul Jung; Mi Kyeong Lee; Yong Moon Lee; Jin Tae Hong; Yeo Pyo Yun; Hwan Soo Yoo

doi:10.1007/s12272-013-0251-y

Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

Dong Mi Shin, Kyeong Mi Choi, Youn Sun Lee, Wonkyun Kim, Kyong Oh Shin, Seikwan Oh, Jae Chul Jung, Mi Kyeong Lee, Yong Moon Lee, Jin Tae Hong, Yeo Pyo Yun, Hwan Soo Yoo

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1- methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.

Original language	English
Pages (from-to)	803-812
Number of pages	10
Journal	Archives of Pharmacal Research
Volume	37
Issue number	6
DOIs	https://doi.org/10.1007/s12272-013-0251-y
State	Published - Jun 2014

Bibliographical note

Funding Information:
Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013320), the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (MRC, 2010-0029480), the Ministry of Trade, Industry & Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-In-dudtry Convergence (BAIO), and the ‘‘Leaders INdustry-university Cooperation’’ Project funded by the Ministry of Education.

Keywords

3T3-L1 cells
Adipocyte differentiation
Dodeca-2(E),4(E)-dienoic acid isobutylamide
Echinacea purpurea
Insulin

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title = "Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells",

abstract = "Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1- methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.",

keywords = "3T3-L1 cells, Adipocyte differentiation, Dodeca-2(E),4(E)-dienoic acid isobutylamide, Echinacea purpurea, Insulin",

author = "Shin, {Dong Mi} and Choi, {Kyeong Mi} and Lee, {Youn Sun} and Wonkyun Kim and Shin, {Kyong Oh} and Seikwan Oh and Jung, {Jae Chul} and Lee, {Mi Kyeong} and Lee, {Yong Moon} and Hong, {Jin Tae} and Yun, {Yeo Pyo} and Yoo, {Hwan Soo}",

note = "Funding Information: Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013320), the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (MRC, 2010-0029480), the Ministry of Trade, Industry & Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-In-dudtry Convergence (BAIO), and the {\textquoteleft}{\textquoteleft}Leaders INdustry-university Cooperation{\textquoteright}{\textquoteright} Project funded by the Ministry of Education.",

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AU - Shin, Dong Mi

AU - Choi, Kyeong Mi

AU - Lee, Youn Sun

AU - Kim, Wonkyun

AU - Shin, Kyong Oh

AU - Oh, Seikwan

AU - Jung, Jae Chul

AU - Lee, Mi Kyeong

AU - Lee, Yong Moon

AU - Hong, Jin Tae

AU - Yun, Yeo Pyo

AU - Yoo, Hwan Soo

N1 - Funding Information: Acknowledgments This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2011-0013320), the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MSIP) (MRC, 2010-0029480), the Ministry of Trade, Industry & Energy (MOTIE, 1415126993) through the fostering project of Osong Academy-In-dudtry Convergence (BAIO), and the ‘‘Leaders INdustry-university Cooperation’’ Project funded by the Ministry of Education.

PY - 2014/6

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N2 - Echinacea purpurea has been shown to have anti-diabetic activities; for example, it activates peroxisome proliferator-activated receptor γ (PPARγ) and increases insulin-stimulated glucose uptake. Adipogenesis has been used to study the insulin signaling pathway and to screen anti-diabetic compounds. The present study was conducted to investigate the effects of an ethanol extract of E. purpurea (EEEP) and its constituents on the insulin-induced adipocyte differentiation of 3T3-L1 preadipocytes. When adipocyte differentiation was induced with insulin plus 3-isobutyl-1- methylxanthine and dexamethasone, the accumulation of lipid droplets and the cellular triglyceride content were significantly increased by EEEP. The expressions of PPARγ and C/EBPα in adipocytes treated with EEEP were gradually increased as compared with control cells. Fat accumulation and triglyceride content of adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly increased as compared with control cells. The expressions of PPARγ and C/EBPα in adipocytes treated with dodeca-2(E),4(E)-dienoic acid isobutylamide were significantly higher than in control cells. These results suggest EEEP promotes the adipogenesis that is partially induced by insulin and that dodeca-2(E),4(E)-dienoic acid isobutylamide appears to be responsible for EEEP-enhanced adipocyte differentiation.

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Echinacea purpurea root extract enhances the adipocyte differentiation of 3T3-L1 cells

Abstract

Bibliographical note

Keywords

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