Dynamic single cell culture array

We demonstrate perfusion culture of arrays of individually trapped cells with dynamic microfluidic control of cellular environment, high maintenance of cell isolation, and low cell death. Hydrodynamically trapped cells were shown to adhere and divide at a comparable rate to cells grown randomly on the same glass substrate. This technique will be useful in quantitative single cell studies that require perfusion of drugs or switching of solutions. Isolated cells in arrays allow facile image processing and eliminate effects of cell-cell communication on results.