Duplex Bioelectronic Tongue for Sensing Umami and Sweet Tastes Based on Human Taste Receptor Nanovesicles

Sae Ryun Ahn, Ji Hyun An, Hyun Seok Song, Jin Wook Park, Sang Hun Lee, Jae Hyun Kim, Jyongsik Jang, Tai Hyun Park

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

For several decades, significant efforts have been made in developing artificial taste sensors to recognize the five basic tastes. So far, the well-established taste sensor is an E-tongue, which is constructed with polymer and lipid membranes. However, the previous artificial taste sensors have limitations in various food, beverage, and cosmetic industries because of their failure to mimic human taste reception. There are many interactions between tastants. Therefore, detecting the interactions in a multiplexing system is required. Herein, we developed a duplex bioelectronic tongue (DBT) based on graphene field-effect transistors that were functionalized with heterodimeric human umami taste and sweet taste receptor nanovesicles. Two types of nanovesicles, which have human T1R1/T1R3 for the umami taste and human T1R2/T1R3 for the sweet taste on their membranes, immobilized on micropatterned graphene surfaces were used for the simultaneous detection of the umami and sweet tastants. The DBT platform led to highly sensitive and selective recognition of target tastants at low concentrations (ca. 100 nM). Moreover, our DBT was able to detect the enhancing effect of taste enhancers as in a human taste sensory system. This technique can be a useful tool for the detection of tastes instead of sensory evaluation and development of new artificial tastants in the food and beverage industry.

Original languageEnglish
Pages (from-to)7287-7296
Number of pages10
JournalACS Nano
Volume10
Issue number8
DOIs
StatePublished - 23 Aug 2016

Bibliographical note

Publisher Copyright:
© 2016 American Chemical Society.

Keywords

  • G-protein coupled receptor (GPCR)
  • bioelectronic tongue
  • duplex taste sensor
  • field-effect transistor (FET)
  • graphene
  • nanovesicle
  • taste receptor

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