Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

Ryan D. Sochol, Daniel Corbett, Sarah Hesse, William E.R. Krieger, Ki Tae Wolf, Minkyu Kim, Kosuke Iwai, Song Li, Luke P. Lee, Liwei Lin

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Continuous flow particulate-based microfluidic processors are in critical demand for emerging applications in chemistry and biology, such as point-of-care molecular diagnostics. Challenges remain, however, for accomplishing biochemical assays in which microparticle immobilization is desired or required during intermediate stages of fluidic reaction processes. Here we present a dual-mode microfluidic reactor that functions autonomously under continuous flow conditions to: (i) execute multi-stage particulate-based fluidic mixing routines, and (ii) array select numbers of microparticles during each reaction stage (e.g., for optical detection). We employ this methodology to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-stage aptamer-based sandwich assay.

Original languageEnglish
Pages (from-to)1405-1409
Number of pages5
JournalLab on a Chip
Volume14
Issue number8
DOIs
StatePublished - 21 Apr 2014

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