Dual effects of NMDA-induced intracellular Ca2+ elevations on cGMP levels in cultured cerebellar granule neurons

Seikwan Oh; Shogo Tokuyama; Patrick P. McCaslin

doi:10.1016/S0306-3623(96)00167-X

Dual effects of NMDA-induced intracellular Ca²⁺ elevations on cGMP levels in cultured cerebellar granule neurons

Seikwan Oh, Shogo Tokuyama, Patrick P. McCaslin

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cyclic GMP (cGMP) levels were markedly elevated by N-methyl-D-aspartate (NMDA) within 1-3 min of incubation, then gradually decreased with incubation time. The NMDA-induced intracellular Ca²⁺ elevations showed maximal levels just after adding NMDA and were maintained for 60 min. NMDA did not show augmentation of cGMP elevation with sodium nitroprusside (SNP), rather it decreased the SNP-induced cGMP elevation after exposure for 60 min. The NMDA-induced elevation of cGMP was remarkably augmented with the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX, 1mM), after 60 min of incubation.

Original language	English
Pages (from-to)	153-157
Number of pages	5
Journal	General Pharmacology
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/S0306-3623(96)00167-X
State	Published - Jan 1997

Keywords

Ca influx
IBMX
NMDA
cGMP
cerebellar granule neurons
phosphodiesterase

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10.1016/S0306-3623(96)00167-X

Cite this

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abstract = "Cyclic GMP (cGMP) levels were markedly elevated by N-methyl-D-aspartate (NMDA) within 1-3 min of incubation, then gradually decreased with incubation time. The NMDA-induced intracellular Ca2+ elevations showed maximal levels just after adding NMDA and were maintained for 60 min. NMDA did not show augmentation of cGMP elevation with sodium nitroprusside (SNP), rather it decreased the SNP-induced cGMP elevation after exposure for 60 min. The NMDA-induced elevation of cGMP was remarkably augmented with the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX, 1mM), after 60 min of incubation.",

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AB - Cyclic GMP (cGMP) levels were markedly elevated by N-methyl-D-aspartate (NMDA) within 1-3 min of incubation, then gradually decreased with incubation time. The NMDA-induced intracellular Ca2+ elevations showed maximal levels just after adding NMDA and were maintained for 60 min. NMDA did not show augmentation of cGMP elevation with sodium nitroprusside (SNP), rather it decreased the SNP-induced cGMP elevation after exposure for 60 min. The NMDA-induced elevation of cGMP was remarkably augmented with the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (IBMX, 1mM), after 60 min of incubation.

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