TY - JOUR
T1 - Double-layered adhesive microneedle bandage based on biofunctionalized mussel protein for cardiac tissue regeneration
AU - Lim, Soomee
AU - Park, Tae Yoon
AU - Jeon, Eun Young
AU - Joo, Kye Il
AU - Cha, Hyung Joon
N1 - Funding Information:
We acknowledge the financial support by the Korea Health Technology R&D Project (grant number: HI20C0090 ) through the Korea Health Industry Development Institute, South Korea funded by the Ministry of Health and Welfare , Korea and the National Research Foundation, South Korea (grant number: NRF-2020M3H4A1A03082879 ) funded by the Ministry of Science and ICT , Korea.
Funding Information:
We acknowledge the financial support by the Korea Health Technology R&D Project (grant number: HI20C0090) through the Korea Health Industry Development Institute, South Korea funded by the Ministry of Health and Welfare, Korea and the National Research Foundation, South Korea (grant number: NRF-2020M3H4A1A03082879) funded by the Ministry of Science and ICT, Korea.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/11
Y1 - 2021/11
N2 - Heart failure following myocardial infarction (MI), the primary cause of mortality worldwide, is the consequence of cardiomyocyte death or dysfunction. Clinical efforts involving the delivery of growth factors (GFs) and stem cells with the aim of regenerating cardiomyocytes for the recovery of structural and functional integrity have largely failed to deliver, mainly due to short half-lives and rapid clearance in in vivo environments. In this work, we selected and genetically fused four biofunctional peptides possessing angiogenic potential, originating from extracellular matrix proteins and GFs, to bioengineered mussel adhesive protein (MAP). We found that MAPs fused with vascular endothelial growth factor (VEGF)-derived peptide and fibronectin-derived RGD peptide significantly promoted the proliferation and migration of endothelial cells in vitro. Based on these characteristics, we fabricated advanced double-layered adhesive microneedle bandages (DL-AMNBs) consisting of a biofunctional MAP-based root and a regenerated silk fibroin (SF)-based tip, allowing homogeneous distribution of the regenerative factor via swellable microneedles. Our developed DL-AMNB system clearly demonstrated better preservation of cardiac muscle and regenerative effects on heart remodeling in a rat MI model, which might be attributed to the prolonged retention of therapeutic peptides as well as secure adhesion between the patch and host myocardium by MAP-inherent strong underwater adhesiveness.
AB - Heart failure following myocardial infarction (MI), the primary cause of mortality worldwide, is the consequence of cardiomyocyte death or dysfunction. Clinical efforts involving the delivery of growth factors (GFs) and stem cells with the aim of regenerating cardiomyocytes for the recovery of structural and functional integrity have largely failed to deliver, mainly due to short half-lives and rapid clearance in in vivo environments. In this work, we selected and genetically fused four biofunctional peptides possessing angiogenic potential, originating from extracellular matrix proteins and GFs, to bioengineered mussel adhesive protein (MAP). We found that MAPs fused with vascular endothelial growth factor (VEGF)-derived peptide and fibronectin-derived RGD peptide significantly promoted the proliferation and migration of endothelial cells in vitro. Based on these characteristics, we fabricated advanced double-layered adhesive microneedle bandages (DL-AMNBs) consisting of a biofunctional MAP-based root and a regenerated silk fibroin (SF)-based tip, allowing homogeneous distribution of the regenerative factor via swellable microneedles. Our developed DL-AMNB system clearly demonstrated better preservation of cardiac muscle and regenerative effects on heart remodeling in a rat MI model, which might be attributed to the prolonged retention of therapeutic peptides as well as secure adhesion between the patch and host myocardium by MAP-inherent strong underwater adhesiveness.
KW - Cardiac tissue regeneration
KW - Double-layered microneedle patch
KW - Functional peptide fusion
KW - Mussel adhesive protein
KW - Myocardial infraction
UR - http://www.scopus.com/inward/record.url?scp=85116244046&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2021.121171
DO - 10.1016/j.biomaterials.2021.121171
M3 - Article
C2 - 34624751
AN - SCOPUS:85116244046
SN - 0142-9612
VL - 278
JO - Biomaterials
JF - Biomaterials
M1 - 121171
ER -