DNA damage in the kidneys of diabetic rats exhibiting microalbuminuria

Hunjoo Ha; Chul Kim; Youngsook Son; Myung Hee Chung; Hwan Kim Kyung Hwan Kim

doi:10.1016/0891-5849(94)90152-X

DNA damage in the kidneys of diabetic rats exhibiting microalbuminuria

Hunjoo Ha, Chul Kim, Youngsook Son, Myung Hee Chung, Hwan Kim Kyung Hwan Kim

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

109 Scopus citations

Abstract

8-Hydroxydeoxyguanosine (8-OHdG), an oxygen radical induced modification of purine residue in DNA, was measured in the liver, pancreas, and kidney of streptozotocin-induced diabetic rats (STZR) exhibiting microalbuminuria. At 4 weeks after the injection of streptozotocin (50 mg/kg, IV), the rate of urinary albumin excretion was 0.5±0.1 and 2.0±0.2 mg/24 h in age-matched control rats (CR) and STZR, respectively. Compared to CR, STZR also showed a significantly increased level of 8-OHdG in the kidney but not the liver and pancreas. Amounts of 8-OHdG/10⁵ dG for CR and STZR were 3.4±0.3 and 5.1±0.2 for renal cortices, and 4.1±0.2 and 20.0±3.7 for renal papillae. Daily injection of insulin (2 U, SC) starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptozotocin rather than a direct nephrotoxic effect of the drug. This study suggests that formation of 8-OHdG and, therefore, oxidative damage are closely related in the process of diabetic nephropathy.

Original language	English
Pages (from-to)	271-274
Number of pages	4
Journal	Free Radical Biology and Medicine
Volume	16
Issue number	2
DOIs	https://doi.org/10.1016/0891-5849(94)90152-X
State	Published - Feb 1994

Bibliographical note

Funding Information:
Acknowledgements -- This work was supported in part by Yuhan Research Fund for the Department of Pharmacology, Yonsei University College of Medicine, by a grant from Korea Science and Engineering Foundation through The Research Center for Cell Differentiation (92-3-2) and by Non-Directed Research Fund from Korea Research Foundation 1992.

Keywords

8-Hydroxydeoxyguanosine (8-OHdG)
Diabetic proteinuria
Free radicals
Insulin
Streptozotocin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0891-5849(94)90152-X

Cite this

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title = "DNA damage in the kidneys of diabetic rats exhibiting microalbuminuria",

abstract = "8-Hydroxydeoxyguanosine (8-OHdG), an oxygen radical induced modification of purine residue in DNA, was measured in the liver, pancreas, and kidney of streptozotocin-induced diabetic rats (STZR) exhibiting microalbuminuria. At 4 weeks after the injection of streptozotocin (50 mg/kg, IV), the rate of urinary albumin excretion was 0.5±0.1 and 2.0±0.2 mg/24 h in age-matched control rats (CR) and STZR, respectively. Compared to CR, STZR also showed a significantly increased level of 8-OHdG in the kidney but not the liver and pancreas. Amounts of 8-OHdG/105 dG for CR and STZR were 3.4±0.3 and 5.1±0.2 for renal cortices, and 4.1±0.2 and 20.0±3.7 for renal papillae. Daily injection of insulin (2 U, SC) starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptozotocin rather than a direct nephrotoxic effect of the drug. This study suggests that formation of 8-OHdG and, therefore, oxidative damage are closely related in the process of diabetic nephropathy.",

keywords = "8-Hydroxydeoxyguanosine (8-OHdG), Diabetic proteinuria, Free radicals, Insulin, Streptozotocin",

author = "Hunjoo Ha and Chul Kim and Youngsook Son and Chung, {Myung Hee} and {Kyung Hwan Kim}, {Hwan Kim}",

note = "Funding Information: Acknowledgements -- This work was supported in part by Yuhan Research Fund for the Department of Pharmacology, Yonsei University College of Medicine, by a grant from Korea Science and Engineering Foundation through The Research Center for Cell Differentiation (92-3-2) and by Non-Directed Research Fund from Korea Research Foundation 1992.",

year = "1994",

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AU - Kim, Chul

AU - Son, Youngsook

AU - Chung, Myung Hee

AU - Kyung Hwan Kim, Hwan Kim

N1 - Funding Information: Acknowledgements -- This work was supported in part by Yuhan Research Fund for the Department of Pharmacology, Yonsei University College of Medicine, by a grant from Korea Science and Engineering Foundation through The Research Center for Cell Differentiation (92-3-2) and by Non-Directed Research Fund from Korea Research Foundation 1992.

PY - 1994/2

Y1 - 1994/2

N2 - 8-Hydroxydeoxyguanosine (8-OHdG), an oxygen radical induced modification of purine residue in DNA, was measured in the liver, pancreas, and kidney of streptozotocin-induced diabetic rats (STZR) exhibiting microalbuminuria. At 4 weeks after the injection of streptozotocin (50 mg/kg, IV), the rate of urinary albumin excretion was 0.5±0.1 and 2.0±0.2 mg/24 h in age-matched control rats (CR) and STZR, respectively. Compared to CR, STZR also showed a significantly increased level of 8-OHdG in the kidney but not the liver and pancreas. Amounts of 8-OHdG/105 dG for CR and STZR were 3.4±0.3 and 5.1±0.2 for renal cortices, and 4.1±0.2 and 20.0±3.7 for renal papillae. Daily injection of insulin (2 U, SC) starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptozotocin rather than a direct nephrotoxic effect of the drug. This study suggests that formation of 8-OHdG and, therefore, oxidative damage are closely related in the process of diabetic nephropathy.

AB - 8-Hydroxydeoxyguanosine (8-OHdG), an oxygen radical induced modification of purine residue in DNA, was measured in the liver, pancreas, and kidney of streptozotocin-induced diabetic rats (STZR) exhibiting microalbuminuria. At 4 weeks after the injection of streptozotocin (50 mg/kg, IV), the rate of urinary albumin excretion was 0.5±0.1 and 2.0±0.2 mg/24 h in age-matched control rats (CR) and STZR, respectively. Compared to CR, STZR also showed a significantly increased level of 8-OHdG in the kidney but not the liver and pancreas. Amounts of 8-OHdG/105 dG for CR and STZR were 3.4±0.3 and 5.1±0.2 for renal cortices, and 4.1±0.2 and 20.0±3.7 for renal papillae. Daily injection of insulin (2 U, SC) starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptozotocin rather than a direct nephrotoxic effect of the drug. This study suggests that formation of 8-OHdG and, therefore, oxidative damage are closely related in the process of diabetic nephropathy.

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DNA damage in the kidneys of diabetic rats exhibiting microalbuminuria

Abstract

Bibliographical note

Keywords

UN SDGs

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