TY - JOUR
T1 - DNA damage in the kidneys of diabetic rats exhibiting microalbuminuria
AU - Ha, Hunjoo
AU - Kim, Chul
AU - Son, Youngsook
AU - Chung, Myung Hee
AU - Kyung Hwan Kim, Hwan Kim
N1 - Funding Information:
Acknowledgements -- This work was supported in part by Yuhan Research Fund for the Department of Pharmacology, Yonsei University College of Medicine, by a grant from Korea Science and Engineering Foundation through The Research Center for Cell Differentiation (92-3-2) and by Non-Directed Research Fund from Korea Research Foundation 1992.
PY - 1994/2
Y1 - 1994/2
N2 - 8-Hydroxydeoxyguanosine (8-OHdG), an oxygen radical induced modification of purine residue in DNA, was measured in the liver, pancreas, and kidney of streptozotocin-induced diabetic rats (STZR) exhibiting microalbuminuria. At 4 weeks after the injection of streptozotocin (50 mg/kg, IV), the rate of urinary albumin excretion was 0.5±0.1 and 2.0±0.2 mg/24 h in age-matched control rats (CR) and STZR, respectively. Compared to CR, STZR also showed a significantly increased level of 8-OHdG in the kidney but not the liver and pancreas. Amounts of 8-OHdG/105 dG for CR and STZR were 3.4±0.3 and 5.1±0.2 for renal cortices, and 4.1±0.2 and 20.0±3.7 for renal papillae. Daily injection of insulin (2 U, SC) starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptozotocin rather than a direct nephrotoxic effect of the drug. This study suggests that formation of 8-OHdG and, therefore, oxidative damage are closely related in the process of diabetic nephropathy.
AB - 8-Hydroxydeoxyguanosine (8-OHdG), an oxygen radical induced modification of purine residue in DNA, was measured in the liver, pancreas, and kidney of streptozotocin-induced diabetic rats (STZR) exhibiting microalbuminuria. At 4 weeks after the injection of streptozotocin (50 mg/kg, IV), the rate of urinary albumin excretion was 0.5±0.1 and 2.0±0.2 mg/24 h in age-matched control rats (CR) and STZR, respectively. Compared to CR, STZR also showed a significantly increased level of 8-OHdG in the kidney but not the liver and pancreas. Amounts of 8-OHdG/105 dG for CR and STZR were 3.4±0.3 and 5.1±0.2 for renal cortices, and 4.1±0.2 and 20.0±3.7 for renal papillae. Daily injection of insulin (2 U, SC) starting on the third day after streptozotocin treatment significantly reduced both urinary albumin excretion and papillary 8-OHdG formation, which suggests that these are associated with the diabetic state induced by streptozotocin rather than a direct nephrotoxic effect of the drug. This study suggests that formation of 8-OHdG and, therefore, oxidative damage are closely related in the process of diabetic nephropathy.
KW - 8-Hydroxydeoxyguanosine (8-OHdG)
KW - Diabetic proteinuria
KW - Free radicals
KW - Insulin
KW - Streptozotocin
UR - http://www.scopus.com/inward/record.url?scp=0028348166&partnerID=8YFLogxK
U2 - 10.1016/0891-5849(94)90152-X
DO - 10.1016/0891-5849(94)90152-X
M3 - Article
C2 - 8005523
AN - SCOPUS:0028348166
SN - 0891-5849
VL - 16
SP - 271
EP - 274
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 2
ER -
