Dissection of the dimerization modes in the DJ-1 superfamily

Hoi Jong Jung, Sangok Kim, Yun Jae Kim, Min Kyu Kim, Sung Gyun Kang, Jung Hyun Lee, Wankyu Kim, Sun Shin Cha

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The DJ-1 superfamily (DJ-1/ThiJ/PfpI superfamily) is distributed across all three kingdoms of life. These proteins are involved in a highly diverse range of cellular functions, including chaperone and protease activity. DJ-1 proteins usually form dimers or hexamers in vivo and show at least four different binding orientations via distinct interface patches. Abnormal oligomerization of human DJ-1 is related to neurodegenerative disorders including Parkinson's disease, suggesting important functional roles of quaternary structures. However, the quaternary structures of the DJ-1 superfamily have not been extensively studied. Here, we focus on the diverse oligomerization modes among the DJ-1 superfamily proteins and investigate the functional roles of quaternary structures both computationally and experimentally. The oligomerization modes are classified into 4 types (DJ-1, YhbO, Hsp, and YDR types) depending on the distinct interface patches (I-IV) upon dimerization. A unique, rotated interface via patch I is reported, which may potentially be related to higher order oligomerization. In general, the groups based on sequence similarity are consistent with the quaternary structural classes, but their biochemical functions cannot be directly inferred using sequence information alone. The observed phyletic pattern suggests the dynamic nature of quaternary structures in the course of evolution. The amino acid residues at the interfaces tend to show lower mutation rates than those of non-interfacial surfaces.

Original languageEnglish
Pages (from-to)163-171
Number of pages9
JournalMolecules and Cells
Issue number2
StatePublished - Feb 2012

Bibliographical note

Funding Information:
S.-S. Cha was supported by the Marine and Extreme Genome Research Center program of the Ministry of Land, Transport and Maritime Affairs, the KORDI in-house program (PE98513), and the Development of Biohydrogen Production Technology Using Hyperthermophilic Archaea program of Ministry of Land, Transport and Maritime Affairs and the National Research Foundation of Korea Grant 2009-0084757. W. Kim was supported by the Ewha Global Top 5 Grant 2011 of Ewha Womans University, by the Global Frontier Project grant (NRF-M1AXA-002-2010-0029763), and by the Basic Science Research Program (2011-0014992) of the National Research Foundation funded by the Ministry of Education, Science and Technology of Korea.


  • DJ-1 superfamily
  • DJ-1/ThiJ/PfpI superfamily
  • Quaternary structure


Dive into the research topics of 'Dissection of the dimerization modes in the DJ-1 superfamily'. Together they form a unique fingerprint.

Cite this