TY - JOUR
T1 - Discovering the Secret of Diseases by Incorporated Tear Exosomes Analysis via Rapid-Isolation System
T2 - ITEARS
AU - Hu, Liang
AU - Zhang, Ting
AU - Ma, Huixiang
AU - Pan, Youjin
AU - Wang, Siyao
AU - Liu, Xiaoling
AU - Dai, Xiaodan
AU - Zheng, Yuyang
AU - Lee, Luke P.
AU - Liu, Fei
N1 - Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.
PY - 2022/8/23
Y1 - 2022/8/23
N2 - Nanoscale small extracellular vesicles (sEVs, exosomes) in tears allow us to investigate the multisignatures of diseases. However, the translations of tear sEVs for biomarker discovery and clinical diagnostics are practically limited by low recovery, long processing time, and small sample volume. Here, we report an incorporated tear-exosomes analysis via rapid-isolation system (iTEARS) via nanotechnology to discover the secrets of ocular disorders and systemic diseases. We isolate exosomes rapidly with high yield and purity from a few teardrops (∼10 μL) within 5 min via nanoporous membrane-based resonators for the quantitative detection and biomarker discovery through proteomic and transcriptomic analysis. We have identified 904 proteins, among which 228 proteins are discovered, 426 proteins are detected from exosomes of dry eye disease, and demonstrate CALML5, KRT6A, and S100P for the classification of dry eye disease. We have also investigated 484 miRNAs in tear exosomes and show miR-145-5p, miR-214-3p, miR-218-5p, and miR-9-5p are dysregulated during diabetic retinopathy development. We believe iTEARS can be used for improving molecular diagnostics via tears to identify ocular disorders, systemic diseases, and numerous other neurodegenerative diseases and cancer.
AB - Nanoscale small extracellular vesicles (sEVs, exosomes) in tears allow us to investigate the multisignatures of diseases. However, the translations of tear sEVs for biomarker discovery and clinical diagnostics are practically limited by low recovery, long processing time, and small sample volume. Here, we report an incorporated tear-exosomes analysis via rapid-isolation system (iTEARS) via nanotechnology to discover the secrets of ocular disorders and systemic diseases. We isolate exosomes rapidly with high yield and purity from a few teardrops (∼10 μL) within 5 min via nanoporous membrane-based resonators for the quantitative detection and biomarker discovery through proteomic and transcriptomic analysis. We have identified 904 proteins, among which 228 proteins are discovered, 426 proteins are detected from exosomes of dry eye disease, and demonstrate CALML5, KRT6A, and S100P for the classification of dry eye disease. We have also investigated 484 miRNAs in tear exosomes and show miR-145-5p, miR-214-3p, miR-218-5p, and miR-9-5p are dysregulated during diabetic retinopathy development. We believe iTEARS can be used for improving molecular diagnostics via tears to identify ocular disorders, systemic diseases, and numerous other neurodegenerative diseases and cancer.
KW - biomarkers
KW - diagnostics
KW - extracellular vesicles
KW - eye diseases
KW - identification
KW - nanotechnology
KW - tears
UR - http://www.scopus.com/inward/record.url?scp=85135954679&partnerID=8YFLogxK
U2 - 10.1021/acsnano.2c02531
DO - 10.1021/acsnano.2c02531
M3 - Article
C2 - 35856505
AN - SCOPUS:85135954679
SN - 1936-0851
VL - 16
SP - 11720
EP - 11732
JO - ACS Nano
JF - ACS Nano
IS - 8
ER -
