TY - JOUR
T1 - Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds
AU - Kwon, Mijung
AU - Bagonis, Maria
AU - Danuser, Gaudenz
AU - Pellman, David
N1 - Funding Information:
We thank M. Thery, B. Akins, H. Arellano-Santoyo, S. Jhaveri-Schneider, and N. Umbreit for comments on the manuscript; L. Ding for sharing computer code; the Nikon Imaging Center at Harvard Medical School and H. Elliott from the Image and Data Analysis Core at Harvard Medical School for their help with kymograph analysis of microtubule dynamics; and R. Cheney, W. Krek, E. Nigg, I. Cheeseman, T. Wittmann, and W. Bement for reagents. D.P. was supported by the Howard Hughes Medical Institute and the NIH (GM061345); M.K. was supported by a special fellow award from the Leukemia and Lymphoma Society and a Susan Komen grant; and M.B. and G.D. were funded by the NIH (GM067230).
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/8/10
Y1 - 2015/8/10
N2 - Positioning of centrosomes is vital for cell division and development. In metazoan cells, spindle positioning is controlled by a dynamic pool of subcortical actin that organizes in response to the position of retraction fibers. These actin "clouds" are proposed to generate pulling forces on centrosomes and mediate spindle orientation. However, the motors that pull astral microtubules toward these actin structures are not known. Here, we report that the unconventional myosin, Myo10, couples actin-dependent forces from retraction fibers and subcortical actin clouds to centrosomes. Myo10-mediated centrosome positioning requires its direct microtubule binding. Computational image analysis of large microtubule populations reveals a direct effect of Myo10 on microtubule dynamics and microtubule-cortex interactions. Myo10's role in centrosome positioning is distinct from, but overlaps with, that of dynein. Thus, Myo10 plays a key role in integrating the actin and microtubule cytoskeletons to position centrosomes and mitotic spindles.
AB - Positioning of centrosomes is vital for cell division and development. In metazoan cells, spindle positioning is controlled by a dynamic pool of subcortical actin that organizes in response to the position of retraction fibers. These actin "clouds" are proposed to generate pulling forces on centrosomes and mediate spindle orientation. However, the motors that pull astral microtubules toward these actin structures are not known. Here, we report that the unconventional myosin, Myo10, couples actin-dependent forces from retraction fibers and subcortical actin clouds to centrosomes. Myo10-mediated centrosome positioning requires its direct microtubule binding. Computational image analysis of large microtubule populations reveals a direct effect of Myo10 on microtubule dynamics and microtubule-cortex interactions. Myo10's role in centrosome positioning is distinct from, but overlaps with, that of dynein. Thus, Myo10 plays a key role in integrating the actin and microtubule cytoskeletons to position centrosomes and mitotic spindles.
UR - http://www.scopus.com/inward/record.url?scp=84939464550&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2015.06.013
DO - 10.1016/j.devcel.2015.06.013
M3 - Article
C2 - 26235048
AN - SCOPUS:84939464550
SN - 1534-5807
VL - 34
SP - 323
EP - 337
JO - Developmental Cell
JF - Developmental Cell
IS - 3
ER -