Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis

Kwiyeom Yoon, Hyun Duk Jang, Soo Young Lee

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16 Scopus citations


Second mitochondria-derived activator of caspase (Smac) has been implicated in the activation of apoptosis in response to cell stress. We screened for Smac/DIABLO-binding protein for further understanding of Smac-mediated apoptosis. We identified NADE, previously known as p75NTR-associated cell death executor, as a Smac-binding protein. Smac-NADE interaction was mapped to the N-terminal region of Samc and the C-terminal region of NADE. Co-expression of NADE and Smac promotes TRAIL-induced apoptosis in MCF-7 cells. Interestingly, the co-presence of Smac and NADE inhibits XIAP-mediated Smac ubiquitination. In conclusion, our results provide the first evidence that the interaction between Smac and NADE regulates apoptosis through the inhibition of Smac ubiquitination.

Original languageEnglish
Pages (from-to)649-654
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - 25 Jun 2004


  • Apoptosis
  • NADE
  • Smac
  • Ubiquitination
  • XIAP


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