Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis

Kwiyeom Yoon; Hyun Duk Jang; Soo Young Lee

doi:10.1016/j.bbrc.2004.05.043

Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis

Kwiyeom Yoon, Hyun Duk Jang, Soo Young Lee

Department of Life Science

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Second mitochondria-derived activator of caspase (Smac) has been implicated in the activation of apoptosis in response to cell stress. We screened for Smac/DIABLO-binding protein for further understanding of Smac-mediated apoptosis. We identified NADE, previously known as p75NTR-associated cell death executor, as a Smac-binding protein. Smac-NADE interaction was mapped to the N-terminal region of Samc and the C-terminal region of NADE. Co-expression of NADE and Smac promotes TRAIL-induced apoptosis in MCF-7 cells. Interestingly, the co-presence of Smac and NADE inhibits XIAP-mediated Smac ubiquitination. In conclusion, our results provide the first evidence that the interaction between Smac and NADE regulates apoptosis through the inhibition of Smac ubiquitination.

Original language	English
Pages (from-to)	649-654
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	319
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2004.05.043
State	Published - 25 Jun 2004

Bibliographical note

Funding Information:
We thank Dr. T. Sato (Columbia University, USA) for NADE expression vectors and Dr. J. Ashwell (NIH, USA) for XIAP expression vectors. This work was supported by Korea Research Foundation Grant (KRF-2000-015-FS0006) (to S.Y.L). K.Y. and H.J. were supported by BK21 fellowship.

Keywords

Apoptosis
NADE
Smac
TRAIL
Ubiquitination
XIAP

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title = "Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis",

abstract = "Second mitochondria-derived activator of caspase (Smac) has been implicated in the activation of apoptosis in response to cell stress. We screened for Smac/DIABLO-binding protein for further understanding of Smac-mediated apoptosis. We identified NADE, previously known as p75NTR-associated cell death executor, as a Smac-binding protein. Smac-NADE interaction was mapped to the N-terminal region of Samc and the C-terminal region of NADE. Co-expression of NADE and Smac promotes TRAIL-induced apoptosis in MCF-7 cells. Interestingly, the co-presence of Smac and NADE inhibits XIAP-mediated Smac ubiquitination. In conclusion, our results provide the first evidence that the interaction between Smac and NADE regulates apoptosis through the inhibition of Smac ubiquitination.",

keywords = "Apoptosis, NADE, Smac, TRAIL, Ubiquitination, XIAP",

author = "Kwiyeom Yoon and Jang, {Hyun Duk} and Lee, {Soo Young}",

note = "Funding Information: We thank Dr. T. Sato (Columbia University, USA) for NADE expression vectors and Dr. J. Ashwell (NIH, USA) for XIAP expression vectors. This work was supported by Korea Research Foundation Grant (KRF-2000-015-FS0006) (to S.Y.L). K.Y. and H.J. were supported by BK21 fellowship.",

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doi = "10.1016/j.bbrc.2004.05.043",

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AU - Jang, Hyun Duk

AU - Lee, Soo Young

N1 - Funding Information: We thank Dr. T. Sato (Columbia University, USA) for NADE expression vectors and Dr. J. Ashwell (NIH, USA) for XIAP expression vectors. This work was supported by Korea Research Foundation Grant (KRF-2000-015-FS0006) (to S.Y.L). K.Y. and H.J. were supported by BK21 fellowship.

PY - 2004/6/25

Y1 - 2004/6/25

N2 - Second mitochondria-derived activator of caspase (Smac) has been implicated in the activation of apoptosis in response to cell stress. We screened for Smac/DIABLO-binding protein for further understanding of Smac-mediated apoptosis. We identified NADE, previously known as p75NTR-associated cell death executor, as a Smac-binding protein. Smac-NADE interaction was mapped to the N-terminal region of Samc and the C-terminal region of NADE. Co-expression of NADE and Smac promotes TRAIL-induced apoptosis in MCF-7 cells. Interestingly, the co-presence of Smac and NADE inhibits XIAP-mediated Smac ubiquitination. In conclusion, our results provide the first evidence that the interaction between Smac and NADE regulates apoptosis through the inhibition of Smac ubiquitination.

AB - Second mitochondria-derived activator of caspase (Smac) has been implicated in the activation of apoptosis in response to cell stress. We screened for Smac/DIABLO-binding protein for further understanding of Smac-mediated apoptosis. We identified NADE, previously known as p75NTR-associated cell death executor, as a Smac-binding protein. Smac-NADE interaction was mapped to the N-terminal region of Samc and the C-terminal region of NADE. Co-expression of NADE and Smac promotes TRAIL-induced apoptosis in MCF-7 cells. Interestingly, the co-presence of Smac and NADE inhibits XIAP-mediated Smac ubiquitination. In conclusion, our results provide the first evidence that the interaction between Smac and NADE regulates apoptosis through the inhibition of Smac ubiquitination.

KW - Apoptosis

KW - NADE

KW - Smac

KW - TRAIL

KW - Ubiquitination

KW - XIAP

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis

Abstract

Bibliographical note

Keywords

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