Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer

Jae Han Kim; Sung Hyeon Lee; Sookyung Choi; Unyong Kim; In Seok Yeo; Su Hee Kim; Myung Jin Oh; Hantae Moon; Jua Lee; Seunghyup Jeong; Min Gew Choi; Jun Ho Lee; Tae Sung Sohn; Jae Moon Bae; Sung Kim; Yang Won Min; Hyuk Lee; Jun Haeng Lee; Poong Lyul Rhee; Jae J. Kim; Su Jin Lee; Seung Tae Kim; Jeeyun Lee; Se Hoon Park; Joon Oh Park; Young Suk Park; Ho Yeong Lim; Won Ki Kang; Hyun Joo An; Jung Hoe Kim

doi:10.18632/oncotarget.14362

Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer

Jae Han Kim, Sung Hyeon Lee, Sookyung Choi, Unyong Kim, In Seok Yeo, Su Hee Kim, Myung Jin Oh, Hantae Moon, Jua Lee, Seunghyup Jeong, Min Gew Choi, Jun Ho Lee, Tae Sung Sohn, Jae Moon Bae, Sung Kim, Yang Won Min, Hyuk Lee, Jun Haeng Lee, Poong Lyul Rhee, Jae J. KimSu Jin Lee, Seung Tae Kim, Jeeyun Lee, Se Hoon Park, Joon Oh Park, Young Suk Park, Ho Yeong Lim, Won Ki Kang, Hyun Joo An, Jung Hoe Kim

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Based on our previous studies, differential analysis of N-glycan expression bound on serum haptoglobin reveals the quantitative variation on gastric cancer patients. In this prospective case-control study, we explore the clinically relevant glycan markers for gastric cancer diagnosis. Serum samples were collected from patients with gastric cancer (n = 44) and healthy control (n = 44). N-glycans alteration was monitored by intact analysis of Hp using liquid chromatography-mass spectrometry followed by immunoaffinity purification with the serum samples. Intensity and frequency markers were defined depending on the mass spectrometry data analysis. Multiple markers were found with high diagnostic efficacy. As intensity markers (I-marker), six markers were discovered with the AUC > 0.8. The high efficiency markers exhibited AUC of 0.93 with a specificity of 86% when the sensitivity was set to 95%. We additionally established frequency marker (f-marker) panels based on the tendency of high N-glycan expression. The AUC to conclude patients and control group were 0.82 and 0.79, respectively. This study suggested that N-glycan variation of serum haptoglobin were associated with patients with gastric cancer and might be a promising marker for the cancer screening.

Original language	English
Pages (from-to)	11094-11104
Number of pages	11
Journal	Oncotarget
Volume	8
Issue number	7
DOIs	https://doi.org/10.18632/oncotarget.14362
State	Published - 2017

Keywords

Biomarker
Glycoprotein
Haptoglobin
N-glycan
Stomach neoplasm

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10.18632/oncotarget.14362

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Kim, J. H., Lee, S. H., Choi, S., Kim, U., Yeo, I. S., Kim, S. H., Oh, M. J., Moon, H., Lee, J., Jeong, S., Choi, M. G., Lee, J. H., Sohn, T. S., Bae, J. M., Kim, S., Min, Y. W., Lee, H., Lee, J. H., Rhee, P. L., ... Kim, J. H. (2017). Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer. Oncotarget, 8(7), 11094-11104. https://doi.org/10.18632/oncotarget.14362

@article{83fcce3b22054ba3aa6a0cd2327a4e1b,

title = "Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer",

abstract = "Based on our previous studies, differential analysis of N-glycan expression bound on serum haptoglobin reveals the quantitative variation on gastric cancer patients. In this prospective case-control study, we explore the clinically relevant glycan markers for gastric cancer diagnosis. Serum samples were collected from patients with gastric cancer (n = 44) and healthy control (n = 44). N-glycans alteration was monitored by intact analysis of Hp using liquid chromatography-mass spectrometry followed by immunoaffinity purification with the serum samples. Intensity and frequency markers were defined depending on the mass spectrometry data analysis. Multiple markers were found with high diagnostic efficacy. As intensity markers (I-marker), six markers were discovered with the AUC > 0.8. The high efficiency markers exhibited AUC of 0.93 with a specificity of 86% when the sensitivity was set to 95%. We additionally established frequency marker (f-marker) panels based on the tendency of high N-glycan expression. The AUC to conclude patients and control group were 0.82 and 0.79, respectively. This study suggested that N-glycan variation of serum haptoglobin were associated with patients with gastric cancer and might be a promising marker for the cancer screening.",

keywords = "Biomarker, Glycoprotein, Haptoglobin, N-glycan, Stomach neoplasm",

author = "Kim, {Jae Han} and Lee, {Sung Hyeon} and Sookyung Choi and Unyong Kim and Yeo, {In Seok} and Kim, {Su Hee} and Oh, {Myung Jin} and Hantae Moon and Jua Lee and Seunghyup Jeong and Choi, {Min Gew} and Lee, {Jun Ho} and Sohn, {Tae Sung} and Bae, {Jae Moon} and Sung Kim and Min, {Yang Won} and Hyuk Lee and Lee, {Jun Haeng} and Rhee, {Poong Lyul} and Kim, {Jae J.} and Lee, {Su Jin} and Kim, {Seung Tae} and Jeeyun Lee and Park, {Se Hoon} and Park, {Joon Oh} and Park, {Young Suk} and Lim, {Ho Yeong} and Kang, {Won Ki} and An, {Hyun Joo} and Kim, {Jung Hoe}",

year = "2017",

doi = "10.18632/oncotarget.14362",

language = "English",

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Kim, JH, Lee, SH, Choi, S, Kim, U, Yeo, IS, Kim, SH, Oh, MJ, Moon, H, Lee, J, Jeong, S, Choi, MG, Lee, JH, Sohn, TS, Bae, JM, Kim, S, Min, YW, Lee, H, Lee, JH, Rhee, PL, Kim, JJ, Lee, SJ, Kim, ST, Lee, J, Park, SH, Park, JO, Park, YS, Lim, HY, Kang, WK, An, HJ & Kim, JH 2017, 'Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer', Oncotarget, vol. 8, no. 7, pp. 11094-11104. https://doi.org/10.18632/oncotarget.14362

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T1 - Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer

AU - Kim, Jae Han

AU - Lee, Sung Hyeon

AU - Choi, Sookyung

AU - Kim, Unyong

AU - Yeo, In Seok

AU - Kim, Su Hee

AU - Oh, Myung Jin

AU - Moon, Hantae

AU - Lee, Jua

AU - Jeong, Seunghyup

AU - Choi, Min Gew

AU - Lee, Jun Ho

AU - Sohn, Tae Sung

AU - Bae, Jae Moon

AU - Kim, Sung

AU - Min, Yang Won

AU - Lee, Hyuk

AU - Lee, Jun Haeng

AU - Rhee, Poong Lyul

AU - Kim, Jae J.

AU - Lee, Su Jin

AU - Kim, Seung Tae

AU - Lee, Jeeyun

AU - Park, Se Hoon

AU - Park, Joon Oh

AU - Park, Young Suk

AU - Lim, Ho Yeong

AU - Kang, Won Ki

AU - An, Hyun Joo

AU - Kim, Jung Hoe

PY - 2017

Y1 - 2017

N2 - Based on our previous studies, differential analysis of N-glycan expression bound on serum haptoglobin reveals the quantitative variation on gastric cancer patients. In this prospective case-control study, we explore the clinically relevant glycan markers for gastric cancer diagnosis. Serum samples were collected from patients with gastric cancer (n = 44) and healthy control (n = 44). N-glycans alteration was monitored by intact analysis of Hp using liquid chromatography-mass spectrometry followed by immunoaffinity purification with the serum samples. Intensity and frequency markers were defined depending on the mass spectrometry data analysis. Multiple markers were found with high diagnostic efficacy. As intensity markers (I-marker), six markers were discovered with the AUC > 0.8. The high efficiency markers exhibited AUC of 0.93 with a specificity of 86% when the sensitivity was set to 95%. We additionally established frequency marker (f-marker) panels based on the tendency of high N-glycan expression. The AUC to conclude patients and control group were 0.82 and 0.79, respectively. This study suggested that N-glycan variation of serum haptoglobin were associated with patients with gastric cancer and might be a promising marker for the cancer screening.

AB - Based on our previous studies, differential analysis of N-glycan expression bound on serum haptoglobin reveals the quantitative variation on gastric cancer patients. In this prospective case-control study, we explore the clinically relevant glycan markers for gastric cancer diagnosis. Serum samples were collected from patients with gastric cancer (n = 44) and healthy control (n = 44). N-glycans alteration was monitored by intact analysis of Hp using liquid chromatography-mass spectrometry followed by immunoaffinity purification with the serum samples. Intensity and frequency markers were defined depending on the mass spectrometry data analysis. Multiple markers were found with high diagnostic efficacy. As intensity markers (I-marker), six markers were discovered with the AUC > 0.8. The high efficiency markers exhibited AUC of 0.93 with a specificity of 86% when the sensitivity was set to 95%. We additionally established frequency marker (f-marker) panels based on the tendency of high N-glycan expression. The AUC to conclude patients and control group were 0.82 and 0.79, respectively. This study suggested that N-glycan variation of serum haptoglobin were associated with patients with gastric cancer and might be a promising marker for the cancer screening.

KW - Biomarker

KW - Glycoprotein

KW - Haptoglobin

KW - N-glycan

KW - Stomach neoplasm

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DO - 10.18632/oncotarget.14362

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AN - SCOPUS:85012935549

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VL - 8

SP - 11094

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ER -

Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer

Abstract

Keywords

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