Dimethylation of H3K4 by Set1 Recruits the Set3 Histone Deacetylase Complex to 5′ Transcribed Regions

Tae Soo Kim; Stephen Buratowski

doi:10.1016/j.cell.2009.02.045

Dimethylation of H3K4 by Set1 Recruits the Set3 Histone Deacetylase Complex to 5′ Transcribed Regions

Tae Soo Kim, Stephen Buratowski

Department of Life Science

Research output: Contribution to journal › Article › peer-review

253 Scopus citations

Abstract

Cotranscriptional histone methylations by Set1 and Set2 have been shown to affect histone acetylation at promoters and 3′ regions of genes, respectively. While histone H3K4 trimethylation (H3K4me3) is thought to promote nucleosome acetylation and remodeling near promoters, we show here that H3K4 dimethylation (H3K4me2) by Set1 leads to reduced histone acetylation levels near 5′ ends of genes. H3K4me2 recruits the Set3 complex via the Set3 PHD finger, localizing the Hos2 and Hst1 subunits to deacetylate histones in 5′ transcribed regions. Cells lacking the Set1-Set3 complex pathway are sensitive to mycophenolic acid and have reduced polymerase levels at a Set3 target gene, suggesting a positive role in transcription. We propose that Set1 establishes two distinct chromatin zones on genes: H3K4me3 leads to high levels of acetylation and low nucleosome density at promoters, while H3K4me2 just downstream recruits the Set3 complex to suppress nucleosome acetylation and remodeling.

Original language	English
Pages (from-to)	259-272
Number of pages	14
Journal	Cell
Volume	137
Issue number	2
DOIs	https://doi.org/10.1016/j.cell.2009.02.045
State	Published - 17 Apr 2009

Bibliographical note

Funding Information:
We thank O. Gozani (Stanford) for histone peptides, S. Briggs (Purdue) for plasmids, L. Howe (University of British Columbia) and M. Keogh (Albert Einstein College of Medicine) for strains, and N. Krogan (University of California, San Francisco), O.Rando (University of Massachusetts, Worcester), and all members of the Buratowski lab for helpful advice and discussions. T.K. is a Special Fellow of the Leukemia and Lymphoma Society. This research was supported by grant GM46498 to S.B. from the United States National Institutes of Health.

Keywords

DNA
PROTEINS

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10.1016/j.cell.2009.02.045

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title = "Dimethylation of H3K4 by Set1 Recruits the Set3 Histone Deacetylase Complex to 5′ Transcribed Regions",

abstract = "Cotranscriptional histone methylations by Set1 and Set2 have been shown to affect histone acetylation at promoters and 3′ regions of genes, respectively. While histone H3K4 trimethylation (H3K4me3) is thought to promote nucleosome acetylation and remodeling near promoters, we show here that H3K4 dimethylation (H3K4me2) by Set1 leads to reduced histone acetylation levels near 5′ ends of genes. H3K4me2 recruits the Set3 complex via the Set3 PHD finger, localizing the Hos2 and Hst1 subunits to deacetylate histones in 5′ transcribed regions. Cells lacking the Set1-Set3 complex pathway are sensitive to mycophenolic acid and have reduced polymerase levels at a Set3 target gene, suggesting a positive role in transcription. We propose that Set1 establishes two distinct chromatin zones on genes: H3K4me3 leads to high levels of acetylation and low nucleosome density at promoters, while H3K4me2 just downstream recruits the Set3 complex to suppress nucleosome acetylation and remodeling.",

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author = "Kim, {Tae Soo} and Stephen Buratowski",

note = "Funding Information: We thank O. Gozani (Stanford) for histone peptides, S. Briggs (Purdue) for plasmids, L. Howe (University of British Columbia) and M. Keogh (Albert Einstein College of Medicine) for strains, and N. Krogan (University of California, San Francisco), O.Rando (University of Massachusetts, Worcester), and all members of the Buratowski lab for helpful advice and discussions. T.K. is a Special Fellow of the Leukemia and Lymphoma Society. This research was supported by grant GM46498 to S.B. from the United States National Institutes of Health. ",

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N2 - Cotranscriptional histone methylations by Set1 and Set2 have been shown to affect histone acetylation at promoters and 3′ regions of genes, respectively. While histone H3K4 trimethylation (H3K4me3) is thought to promote nucleosome acetylation and remodeling near promoters, we show here that H3K4 dimethylation (H3K4me2) by Set1 leads to reduced histone acetylation levels near 5′ ends of genes. H3K4me2 recruits the Set3 complex via the Set3 PHD finger, localizing the Hos2 and Hst1 subunits to deacetylate histones in 5′ transcribed regions. Cells lacking the Set1-Set3 complex pathway are sensitive to mycophenolic acid and have reduced polymerase levels at a Set3 target gene, suggesting a positive role in transcription. We propose that Set1 establishes two distinct chromatin zones on genes: H3K4me3 leads to high levels of acetylation and low nucleosome density at promoters, while H3K4me2 just downstream recruits the Set3 complex to suppress nucleosome acetylation and remodeling.

AB - Cotranscriptional histone methylations by Set1 and Set2 have been shown to affect histone acetylation at promoters and 3′ regions of genes, respectively. While histone H3K4 trimethylation (H3K4me3) is thought to promote nucleosome acetylation and remodeling near promoters, we show here that H3K4 dimethylation (H3K4me2) by Set1 leads to reduced histone acetylation levels near 5′ ends of genes. H3K4me2 recruits the Set3 complex via the Set3 PHD finger, localizing the Hos2 and Hst1 subunits to deacetylate histones in 5′ transcribed regions. Cells lacking the Set1-Set3 complex pathway are sensitive to mycophenolic acid and have reduced polymerase levels at a Set3 target gene, suggesting a positive role in transcription. We propose that Set1 establishes two distinct chromatin zones on genes: H3K4me3 leads to high levels of acetylation and low nucleosome density at promoters, while H3K4me2 just downstream recruits the Set3 complex to suppress nucleosome acetylation and remodeling.

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Dimethylation of H3K4 by Set1 Recruits the Set3 Histone Deacetylase Complex to 5′ Transcribed Regions

Abstract

Bibliographical note

Keywords

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