Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media

Chung Jong Kim, Nak Hyun Kim, Kyoung Ho Song, Pyoeng Gyun Choe, Eu Suk Kim, Sang Won Park, Hong Bin Kim, Nam Joong Kim, Eui Chong Kim, Wan Beom Park, Myoung don Oh

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Nontuberculous mycobacteria (NTM) are classified into 2 categories: slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM), based on interval to colony formation by subculture on solid media. However, little is known about the growth rate of NTM in liquid broth media. We evaluated the differences in time to growth detection (TGD) of RGM and SGM in liquid broth media according to acid-fast stain. Among the 696 NTM isolates, 201 were RGM and 495 were SGM. In acid-fast bacilli (AFB)-negative specimens, the mean TGD was 133 h for RGM and 269 h for SGM (P < 0.001). In AFB-positive specimens, the mean TGD was 112 ± 37 h for RGM and 155 ± 125 h for SGM (P = 0.063). In the AFB-negative group, a cut-off value of 6 days was most effective for distinguishing SGM from RGM; however, in the AFB-positive group, an appropriate cut-off value was hard to define with TGD only.

Original languageEnglish
Pages (from-to)73-76
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume75
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Acid-fast bacilli
  • Atypical mycobacteria
  • Rapid-growing mycobacteria
  • Slow-growing mycobacteria
  • Time to growth detection

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