Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media

Chung Jong Kim; Nak Hyun Kim; Kyoung Ho Song; Pyoeng Gyun Choe; Eu Suk Kim; Sang Won Park; Hong Bin Kim; Nam Joong Kim; Eui Chong Kim; Wan Beom Park; Myoung don Oh

doi:10.1016/j.diagmicrobio.2012.09.019

Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media

Chung Jong Kim, Nak Hyun Kim, Kyoung Ho Song, Pyoeng Gyun Choe, Eu Suk Kim, Sang Won Park, Hong Bin Kim, Nam Joong Kim, Eui Chong Kim, Wan Beom Park, Myoung don Oh

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Nontuberculous mycobacteria (NTM) are classified into 2 categories: slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM), based on interval to colony formation by subculture on solid media. However, little is known about the growth rate of NTM in liquid broth media. We evaluated the differences in time to growth detection (TGD) of RGM and SGM in liquid broth media according to acid-fast stain. Among the 696 NTM isolates, 201 were RGM and 495 were SGM. In acid-fast bacilli (AFB)-negative specimens, the mean TGD was 133 h for RGM and 269 h for SGM (P < 0.001). In AFB-positive specimens, the mean TGD was 112 ± 37 h for RGM and 155 ± 125 h for SGM (P = 0.063). In the AFB-negative group, a cut-off value of 6 days was most effective for distinguishing SGM from RGM; however, in the AFB-positive group, an appropriate cut-off value was hard to define with TGD only.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	75
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2012.09.019
State	Published - Jan 2013

Keywords

Acid-fast bacilli
Atypical mycobacteria
Rapid-growing mycobacteria
Slow-growing mycobacteria
Time to growth detection

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.diagmicrobio.2012.09.019

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title = "Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media",

abstract = "Nontuberculous mycobacteria (NTM) are classified into 2 categories: slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM), based on interval to colony formation by subculture on solid media. However, little is known about the growth rate of NTM in liquid broth media. We evaluated the differences in time to growth detection (TGD) of RGM and SGM in liquid broth media according to acid-fast stain. Among the 696 NTM isolates, 201 were RGM and 495 were SGM. In acid-fast bacilli (AFB)-negative specimens, the mean TGD was 133 h for RGM and 269 h for SGM (P < 0.001). In AFB-positive specimens, the mean TGD was 112 ± 37 h for RGM and 155 ± 125 h for SGM (P = 0.063). In the AFB-negative group, a cut-off value of 6 days was most effective for distinguishing SGM from RGM; however, in the AFB-positive group, an appropriate cut-off value was hard to define with TGD only.",

keywords = "Acid-fast bacilli, Atypical mycobacteria, Rapid-growing mycobacteria, Slow-growing mycobacteria, Time to growth detection",

author = "Kim, {Chung Jong} and Kim, {Nak Hyun} and Song, {Kyoung Ho} and Choe, {Pyoeng Gyun} and Kim, {Eu Suk} and Park, {Sang Won} and Kim, {Hong Bin} and Kim, {Nam Joong} and Kim, {Eui Chong} and Park, {Wan Beom} and Oh, {Myoung don}",

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AU - Kim, Chung Jong

AU - Kim, Nak Hyun

AU - Song, Kyoung Ho

AU - Choe, Pyoeng Gyun

AU - Kim, Eu Suk

AU - Park, Sang Won

AU - Kim, Hong Bin

AU - Kim, Nam Joong

AU - Kim, Eui Chong

AU - Park, Wan Beom

AU - Oh, Myoung don

PY - 2013/1

Y1 - 2013/1

N2 - Nontuberculous mycobacteria (NTM) are classified into 2 categories: slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM), based on interval to colony formation by subculture on solid media. However, little is known about the growth rate of NTM in liquid broth media. We evaluated the differences in time to growth detection (TGD) of RGM and SGM in liquid broth media according to acid-fast stain. Among the 696 NTM isolates, 201 were RGM and 495 were SGM. In acid-fast bacilli (AFB)-negative specimens, the mean TGD was 133 h for RGM and 269 h for SGM (P < 0.001). In AFB-positive specimens, the mean TGD was 112 ± 37 h for RGM and 155 ± 125 h for SGM (P = 0.063). In the AFB-negative group, a cut-off value of 6 days was most effective for distinguishing SGM from RGM; however, in the AFB-positive group, an appropriate cut-off value was hard to define with TGD only.

AB - Nontuberculous mycobacteria (NTM) are classified into 2 categories: slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM), based on interval to colony formation by subculture on solid media. However, little is known about the growth rate of NTM in liquid broth media. We evaluated the differences in time to growth detection (TGD) of RGM and SGM in liquid broth media according to acid-fast stain. Among the 696 NTM isolates, 201 were RGM and 495 were SGM. In acid-fast bacilli (AFB)-negative specimens, the mean TGD was 133 h for RGM and 269 h for SGM (P < 0.001). In AFB-positive specimens, the mean TGD was 112 ± 37 h for RGM and 155 ± 125 h for SGM (P = 0.063). In the AFB-negative group, a cut-off value of 6 days was most effective for distinguishing SGM from RGM; however, in the AFB-positive group, an appropriate cut-off value was hard to define with TGD only.

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KW - Slow-growing mycobacteria

KW - Time to growth detection

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Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media

Abstract

Keywords

UN SDGs

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