Differential expression of protein kinase C isoforms in diethylnitrosamine-initiated rat liver

Yun Sil Lee, Seok Il Hong, Min Jae Lee, Mee Rhan Kim, Ja June Jang

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Although protein kinase C (PKC), a family of 12 related isoforms, plays an important role in carcinogenesis, little is known about the specific role of each isoform in the initiation stage of hepatocarcinogenesis. The subcellular distribution of PKC isoforms in the early stages of diethylnitrosamine (DEN)-initiated hepatocarcinogenesis was therefore examined. Three-week-old female Sprague-Dawley rats were intraperitoneally injected twice in 1 week with DEN; all animals were sacrificed at 1, 2 and 24 h and 3 and 7 days after the second injection. PKCα and -β expression in both cytosolic and particulate fractions decreased as a result of 1 h of DEN treatment and this effect lasted for 7 days. In both fractions, PKCε expression showed a marked increase by DEN treatment, while the expression of PKCδ and -ζ was almost unchanged. These results suggest that differential expression of PKC isoforms may play an important role in the early stage of DEN-initiated hepatocarcinogenesis in rats.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalCancer Letters
Issue number1
StatePublished - 1 Apr 1998

Bibliographical note

Funding Information:
This study was supported by grants from the Academic Research Fund of the Ministry of Education, Republic of Korea and from the Korea Science and Engineering Foundation (KOSEF), through the Cancer Research Center at Seoul National University. We are grateful to Mr Kyung-Joong Kim for his excellent technical assistance.


  • Diethylnitrosamine
  • Initiation
  • Liver
  • Protein kinase C isoforms
  • Rat


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