AKR/J mice carrying leukemia viral inserts develop thymic lymphoma. Recently, we demonstrated that the incidence of thymic lymphoma was decreased when these mice were raised in a low-dose-rate γ-irradiation facility. In contrast, mice irradiated at a high-dose rate developed severe thymic lymphoma and died much earlier. To understand the genetic changes occurred by low- versus high-dose-rate γ-irradiation whole genome microarray was performed. Both groups of mice demonstrated up-regulation of Ifng, Igbp1, and IL7 in their thymuses, however, mice exposed to high-dose-rate γ-irradiation exhibited marked down-regulation of Sp3, Il15, Traf6, IL2ra, Pik3r1, and Hells. In contrast, low-dose-rate irradiated mice demonstrated up-regulation of Il15 and Jag2. These gene expression profiles imply the impaired immune signaling pathways by high-dose-rate γ-irradiation while the facilitation of anti-tumor immune responses by low-dose-rate γ-irradiation. Therefore, our data delineate common and distinct immune-associated pathways downstream of low- versus high-dose-rate irradiation in the process of cancer progression in AKR/J mice.
Bibliographical noteFunding Information:
This work was supported by a grant from the ministry of Knowledge and Economy, Republic of Korea and from Korea Hydro and Nuclear Power Co., Ltd ( 2010T100100303 ). K.-M. Lee is supported by a grant from KICOS ( K20704000007-10A0500-00710 ) and from the National Nuclear R and D program (Grant BAERI) . K. Kim is supported by K20902001448-10E0100-03010 . S.A. Lim is supported by 2010K001437.
- AKR/J mice
- Immune-associated genes
- Low-dose-rate irradiation
- Thymic lymphoma