Different induction of adaptive response to ionizing radiation in normal and neoplastic cells

S. H. Park, Y. Lee, K. Jeong, S. Y. Yoo, C. K. Cho, Yun Sil Lee

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Since the beneficial effects of low-dose radiation (0.01 Gy) are usually observed in normal cells, we investigated whether the adaptive response was induced by low-dose radiation in neoplastic cells of different origin as well as in normal cells. Cell lines used in this experiment were as follows: mouse lymphocytes (NL); L929 cells established from mouse connective tissue; primary mouse keratinocytes (PK); line 308 from mouse papilloma; X-ray sensitive lymphoma cells, L5178Y-S and EL-4 cells from mouse lymphoma. The adaptive response was determined by cell survival and apoptosis. The involvement of apoptosis in the adaptive response was examined by ELISA and TUNEL assay. Adaptive response was induced by pretreatment with low-dose radiation of 0.01 Gy in normal cells such as NL, L929, and PK, but not in L5178Y-S, EL-4, and line 308 cells. In addition, the reduction of apoptosis by pretreatment with low-dose radiation was observed in NL, L929, and PK, but not in L5178Y-S, EL-4, and line 308 cells. These results suggested that the adaptive response could be induced by pretreatment with low-dose radiation and the phenomena were observed in normal cells, not in neoplastic cells. In addition, pretreatment with low-dose radiation reduced apoptosis, suggesting that an anti-apoptotic pathway may be involved in the adaptive response.

Original languageEnglish
Pages (from-to)111-119
Number of pages9
JournalCell Biology and Toxicology
Volume15
Issue number2
DOIs
StatePublished - 1999

Keywords

  • Adaptive response
  • Apoptosis
  • Low-dose radiation
  • Neoplastic cells
  • Normal cells

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