TY - JOUR
T1 - Different induction of adaptive response to ionizing radiation in normal and neoplastic cells
AU - Park, S. H.
AU - Lee, Y.
AU - Jeong, K.
AU - Yoo, S. Y.
AU - Cho, C. K.
AU - Lee, Yun Sil
N1 - Funding Information:
We thank Y. H. Ji and D. H. Lee at the Department of Therapeutic Radiology, Korea Cancer Center Hospital for having irradiated the cells used in this study, and also thank K. J. Kim for his excellent technical assistance. Our work was supported by a National Project Grant from the Ministry of Science and Technology.
PY - 1999
Y1 - 1999
N2 - Since the beneficial effects of low-dose radiation (0.01 Gy) are usually observed in normal cells, we investigated whether the adaptive response was induced by low-dose radiation in neoplastic cells of different origin as well as in normal cells. Cell lines used in this experiment were as follows: mouse lymphocytes (NL); L929 cells established from mouse connective tissue; primary mouse keratinocytes (PK); line 308 from mouse papilloma; X-ray sensitive lymphoma cells, L5178Y-S and EL-4 cells from mouse lymphoma. The adaptive response was determined by cell survival and apoptosis. The involvement of apoptosis in the adaptive response was examined by ELISA and TUNEL assay. Adaptive response was induced by pretreatment with low-dose radiation of 0.01 Gy in normal cells such as NL, L929, and PK, but not in L5178Y-S, EL-4, and line 308 cells. In addition, the reduction of apoptosis by pretreatment with low-dose radiation was observed in NL, L929, and PK, but not in L5178Y-S, EL-4, and line 308 cells. These results suggested that the adaptive response could be induced by pretreatment with low-dose radiation and the phenomena were observed in normal cells, not in neoplastic cells. In addition, pretreatment with low-dose radiation reduced apoptosis, suggesting that an anti-apoptotic pathway may be involved in the adaptive response.
AB - Since the beneficial effects of low-dose radiation (0.01 Gy) are usually observed in normal cells, we investigated whether the adaptive response was induced by low-dose radiation in neoplastic cells of different origin as well as in normal cells. Cell lines used in this experiment were as follows: mouse lymphocytes (NL); L929 cells established from mouse connective tissue; primary mouse keratinocytes (PK); line 308 from mouse papilloma; X-ray sensitive lymphoma cells, L5178Y-S and EL-4 cells from mouse lymphoma. The adaptive response was determined by cell survival and apoptosis. The involvement of apoptosis in the adaptive response was examined by ELISA and TUNEL assay. Adaptive response was induced by pretreatment with low-dose radiation of 0.01 Gy in normal cells such as NL, L929, and PK, but not in L5178Y-S, EL-4, and line 308 cells. In addition, the reduction of apoptosis by pretreatment with low-dose radiation was observed in NL, L929, and PK, but not in L5178Y-S, EL-4, and line 308 cells. These results suggested that the adaptive response could be induced by pretreatment with low-dose radiation and the phenomena were observed in normal cells, not in neoplastic cells. In addition, pretreatment with low-dose radiation reduced apoptosis, suggesting that an anti-apoptotic pathway may be involved in the adaptive response.
KW - Adaptive response
KW - Apoptosis
KW - Low-dose radiation
KW - Neoplastic cells
KW - Normal cells
UR - http://www.scopus.com/inward/record.url?scp=0033002298&partnerID=8YFLogxK
U2 - 10.1023/A:1007525531145
DO - 10.1023/A:1007525531145
M3 - Article
C2 - 10408358
AN - SCOPUS:0033002298
VL - 15
SP - 111
EP - 119
JO - Cell Biology and Toxicology
JF - Cell Biology and Toxicology
SN - 0742-2091
IS - 2
ER -