Dietary calcium and 1,25-dihydroxyvitamin D3 regulate transcription of calcium transporter genes in calbindin-D9k knockout mice

Sang Hwan Ko, Geun Shik Lee, Thuy T.B. Vo, Eui Man Jung, Kyung Chul Choi, Ki Wha Cheung, Jae Wha Kim, Jong Gil Park, Goo Taeg Oh, Eui Bae Jeung

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The effect(s) of oral calcium and vitamin D3 were examined on the expression of duodenal and renal active calcium transport genes, i.e., calbindin-D9k (CaBP-9k) and calbindin-D28k (CaBP-28k), transient receptor potential cation channels (TRPV5 and TRPV6), Na+/Ca2+ exchanger 1 (NCX1) and plasma membrane calcium ATPase 1b (PMCA1b), in CaBP-9k KO mice. Wild-type (WT) and KO mice were provided with calcium and vitamin D3-deficient diets for 10 weeks. The deficient diet significantly decreased body weights compared with the normal diet groups. The serum calcium concentration of the WT mice was decreased by the deficient diet but was unchanged in the KO mice. The deficient diet significantly increased duodenal transcription of CaBP-9k and TRPV6 in the WT mice, but no alteration was observed in the KO mice. In the kidney, the deficient diet significantly increased renal transcripts of CaBP-9k, TRPV6, PMCA1b, CaBP-28k and TRPV5 in the WT mice but did not alter calcium-relating genes in the KO mice. Two potential mediators of calcium-processing genes, vitamin D receptor (VDR) and parathyroid hormone receptor (PTHR), have been suggested to be useful for elucidating these differential regulations in the calcium-related genes of the KO mice. Expression of VDR was not significantly affected by diet or the KO mutation. Renal PTHR mRNA levels were reduced by the diet, and reduced expression was also seen in the KO mice given the normal diet. Taken together, these results suggest that the active calcium transporting genes in KO mice may have resistance to the deficiency diet of calcium and vitamin D3.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalJournal of Reproduction and Development
Volume55
Issue number2
DOIs
StatePublished - 13 May 2009

Keywords

  • Calbindin-D28k (CaBP-28k)
  • Calbindin-D9k (CaBP-9k)
  • Knokout mouse
  • Na/Ca exchanger 1 (NCX1)
  • Plasma membrane calcium ATPase 1b (PMCA1b)
  • TRPV5
  • TRPV6

Fingerprint

Dive into the research topics of 'Dietary calcium and 1,25-dihydroxyvitamin D3 regulate transcription of calcium transporter genes in calbindin-D9k knockout mice'. Together they form a unique fingerprint.

Cite this