Dibutyryl cGMP augments kainate-induced elevation of intracellular calcium levels but not extracellular glutamate in cultured cerebellar granule neurons

Seikwan Oh, Zhengwei Cai, Patrick P. McCaslin

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

These studies were designed to examine the effect of cGMP on glutamate neurotransmission. In primary cultures of cerebellar granule cells, the glutamate receptor agonists, N-methyl-D-aspartate (NMDA) and kainate, stimulate an elevation of intracellular calcium ([Ca2+](i)), the release of glutamate, the synthesis of nitric oxide (NO) and an increase in cGMP. Although it is clear that NMDA- and kainate-induced NO stimulates guanylyl cyclase, resulting in elevation of cGMP, it is unclear whether cGMP augments the NMDA-induced elevations of extracellular glutamate or [Ca2+](i) levels. We show that the membrane permeable cGMP analogue, dibutyryl cGMP (dBcGMP), slightly augments the NMDA-induced elevation of [Ca2+](i) and significantly augments the kainate-induced the elevation of [Ca2+](i) but augments neither NMDA- nor kainate-induced release of glutamate. These results suggest that cGMP has a role in maintaining the kainate and partially NMDA receptor activation. cGMP may not be involved in EAA-induced glutamate release.

Original languageEnglish
Pages (from-to)821-823
Number of pages3
JournalMedical Science Research
Volume24
Issue number12
StatePublished - 1996

Keywords

  • Ca influx
  • Cerebellar granule neurons
  • Dibutyryl cGMP (dBcGMP)
  • Glutamate release

Fingerprint

Dive into the research topics of 'Dibutyryl cGMP augments kainate-induced elevation of intracellular calcium levels but not extracellular glutamate in cultured cerebellar granule neurons'. Together they form a unique fingerprint.

Cite this