DhITACT: DNA hydrogel formation by isothermal amplification of complementary target in fluidic channels

Ho Yeon Lee; Hansaem Jeong; Il Young Jung; Bora Jang; Young Chang Seo; Haeshin Lee; Hyukjin Lee

doi:10.1002/adma.201500414

DhITACT: DNA hydrogel formation by isothermal amplification of complementary target in fluidic channels

Ho Yeon Lee, Hansaem Jeong, Il Young Jung, Bora Jang, Young Chang Seo, Haeshin Lee, Hyukjin Lee

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

DNA hydrogel formation by isothermal amplification of complementary targets in microfluidic channels (DhITACT) is a new platform for rapid and accurate detection of infectious pathogens. DNA hydrogel is formed in situ within microfluidic channels by the isothermal rolling circle amplification process upon the selective binding of target strands from the biological fluid. Once the volume of DNA hydrogel sufficiently enlarges, it can selectively block the matching channels with target pathogens.

Original language	English
Pages (from-to)	3513-3517
Number of pages	5
Journal	Advanced Materials
Volume	27
Issue number	23
DOIs	https://doi.org/10.1002/adma.201500414
State	Published - 1 Jun 2015

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

DNA hydrogel
microfluidic array chip
molecular diagnostic platform
rolling circle amplification

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10.1002/adma.201500414

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abstract = "DNA hydrogel formation by isothermal amplification of complementary targets in microfluidic channels (DhITACT) is a new platform for rapid and accurate detection of infectious pathogens. DNA hydrogel is formed in situ within microfluidic channels by the isothermal rolling circle amplification process upon the selective binding of target strands from the biological fluid. Once the volume of DNA hydrogel sufficiently enlarges, it can selectively block the matching channels with target pathogens.",

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AU - Jeong, Hansaem

AU - Jung, Il Young

AU - Jang, Bora

AU - Seo, Young Chang

AU - Lee, Haeshin

AU - Lee, Hyukjin

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KW - rolling circle amplification

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DhITACT: DNA hydrogel formation by isothermal amplification of complementary target in fluidic channels

Abstract

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Keywords

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