Dextran sulfate-coated superparamagnetic iron oxide nanoparticles as a contrast agent for atherosclerosis imaging

Dong Gil You, Gurusamy Saravanakumar, Soyoung Son, Hwa Seung Han, Roun Heo, Kwangmeyung Kim, Ick Chan Kwon, Jun Young Lee, Jae Hyung Park

Research output: Contribution to journalArticlepeer-review

82 Scopus citations


The hallmark of atherosclerosis in its early pathogenic process is the overexpression of class A scavenger receptors (SR-A) by activated macrophages. In this study, dextran sulfate-coated superparamagnetic iron oxide nanoparticles (DS-SPIONs), as a magnetic resonance (MR) imaging contrast agent of atherosclerosis, was prepared via the facile co-precipitation method using a versatile double-hydrophilic block copolymer comprising of a DS segment (ligand for SR-A) and a poly(glyclerol methacrylate) segment (SPIONs surface-anchoring unit). The physicochemical properties of the DS-SPIONs were investigated using various instruments. DS-SPIONs exhibited high aqueous stability compared to dextran-coated SPIONs (Dex-SPIONs), which were used as controls. The cellular uptake behaviors of DS-SPIONs and Dex-SPIONs were evaluated using Prussian blue assay. Interestingly, the DS-SPIONs were effectively taken up by activated macrophages compared to Dex-SPIONs. However, the cellular uptake of DS-SPIONs by activated macrophages was remarkably reduced in the presence of free DS. These results suggest that activated macrophages internalize DS-SPIONs via receptor (SR-A)-mediated endocytosis. T2-weighted MR imaging of the cells demonstrated that activated macrophages treated with DS-SPIONs showed a significantly lower signal intensity compared to those treated with Dex-SPIONs. Overall, these results suggest that DS-SPIONs may be utilized as a potential contrast agent for atherosclerosis MR imaging.

Original languageEnglish
Pages (from-to)1225-1233
Number of pages9
JournalCarbohydrate Polymers
Issue number1
StatePublished - 2014

Bibliographical note

Funding Information:
This work was financially supported by the Basic Science Research Programs ( 20100027955 & 2012012827 ) of MEST.


  • Atherosclerosis
  • Contrast agent
  • Dextran sulfate
  • Double hydrophilic copolymer
  • Magnetic resonance imaging


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