Development of ultrasound aided chemical pretreatment methods to enrich saccharification of wheat waste biomass for polyhydroxybutyrate production and its characterization

Ganesh Dattatraya Saratale, Rijuta Ganesh Saratale, Sunita Varjani, Si Kyung Cho, Gajanan S. Ghodake, Avinash Kadam, Sikandar I. Mulla, Ram Naresh Bharagava, Dong Su Kim, Han Seung Shin

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64 Scopus citations


Development of an effective pretreatment method is a vital stage to enhance biomass-based biopolymers production. The present study aims on the pretreatment of wheat waste biomass (WS) with alkaline and in combination with ultrasound (US) treatment to enhance enzymatic saccharification of WS into monomeric sugars. Effects of chemical dosage, substrate loading, US power and US pretreatment time on the pretreatment efficiency were systematically investigated. Under optimized conditions; US + NaOH pretreatment attained maximum 70% delignification of WS, with 84.5% hydrolysis yield, 90% glucose yield and 65% xylose yield after enzymatic hydrolysis (30 FPU/g of dry WS) which is substantially higher than the individual pretreatment. Analytical studies (XRD, FTIR, and SEM) revealed that the combined US + NaOH pretreatment can effectively destroyed the ultrastructure of biomass and improved the accessibility to hydrolyzing enzymes. The resulted enzymatic hydrolysates were utilized as a possible biomass feedstock for polyhydroxybutyrate (PHB) production using Ralstonia eutropha. Effects of supplementation of nutrients, stressing agents and initial concentration of US + NaOH pretreated WS hydrolysates (20 to 40 g/L) on PHB production were evaluated. The maximum 74% PHA accumulation, PHB titer of 7.85 g/L and yield of PHB about 0.441 g/g of reducing sugar production was obtained. Analytical characterization and thermal characteristics of extracted biopolymer showed the poperies of standard PHB. The foregoing results suggested the potential of WS as an alternate renewable resource for sustainable biopolymer production and enables WS waste biomass disposal concerns.

Original languageEnglish
Article number112425
JournalIndustrial Crops and Products
StatePublished - Aug 2020

Bibliographical note

Funding Information:
This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) ( 319049-3 ) and funded by Korea Environmental Industry & Technology Institute ( A117-00197-0703-0 ). Authors GDS and RGS are thankful to Dongguk University, Seoul, South Korea under research fund 2018–2020.

Publisher Copyright:
© 2020 Elsevier B.V.


  • Biomass structure
  • Enzymatic hydrolysis
  • Poly(3-hydroxybutyrate)
  • Ralstonia eutropha (ATCC 17699)
  • Ultrasound pretreatment
  • Wheat waste biomass


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