Development of simultaneous analysis of tryptophan metabolites in serum and gastric juice – an investigation towards establishing a biomarker test for gastric cancer diagnosis

Jong Min Choi, Won Sang Park, Kyo Young Song, Hwa Jeong Lee, Byung Hwa Jung

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

To evaluate changes in tryptophan metabolism and discover diagnostic biomarkers for gastric cancer, a quantitative method was developed for tryptophan and its seven metabolites (indole-3-lactic acid, anthranilic acid, serotonin, nicotinic acid, kynurenic acid, kynurenine and 3-indoxyl sulfate) in both human serum and gastric juice using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Serum and gastric juice were prepared with a simple protein precipitation using aqueous 0.1% formic acid and acetonitrile. As a result, it was found that the kynurenine pathway of tryptophan metabolism was activated in gastric cancer and that the metabolic ratio of kynurenine/tryptophan, which reflects the enzyme activity of indoleamine-2,3-dioxygenase, was associated with the observed metabolic changes. Finally, the investigation of tryptophan metabolites, especially kynurenic acid, in serum and gastric juice might serve as biomarkers for gastric cancer. The findings in this study provide critical information of tryptophan metabolism which can be applied to a serum-based diagnostic test for gastric cancer.

Original languageEnglish
Pages (from-to)1963-1974
Number of pages12
JournalBiomedical Chromatography
Volume30
Issue number12
DOIs
StatePublished - 1 Dec 2016

Keywords

  • gastric cancer
  • gastric juice
  • liquid chromatography–tandem mass spectrometry
  • metabolite
  • serum
  • tryptophan

Fingerprint

Dive into the research topics of 'Development of simultaneous analysis of tryptophan metabolites in serum and gastric juice – an investigation towards establishing a biomarker test for gastric cancer diagnosis'. Together they form a unique fingerprint.

Cite this