Development of rice bran treatment process and its use for the synthesis of polyhydroxyalkanoates from rice bran hydrolysate solution

Young Hoon Oh, Seung Hwan Lee, Young Ah Jang, Jae Woo Choi, Kyung Sik Hong, Ju Hyun Yu, Jihoon Shin, Bong Keun Song, Shaik G. Mastan, Yokimiko David, Mary Grace Baylon, Sang Yup Lee, Si Jae Park

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Rice bran treatment process for the production of 43.7 kg of hydrolysate solution containing 24.41 g/L of glucose and small amount of fructose from 5 kg of rice bran was developed and employed to produce polyhydroxyalkanoates in recombinant Escherichia coli and Ralstonia eutropha strains. Recombinant E. coli XL1-Blue expressing R. eutropha phaCAB genes and R. eutropha NCIMB11599 could produce poly(3-hydroxybutyrate) with the polymer contents of 90.1 wt% and 97.2 wt%, respectively, when they were cultured in chemically defined MR medium and chemically defined nitrogen free MR medium containing 10. mL/L of rice bran hydrolysate solution, respectively. Also, recombinant E. coli XL1-Blue and recombinant R. eutropha 437-540, both of which express the Pseudomonas sp. phaC1437 gene and the Clostridium propionicum pct540 gene could produce poly(3-hydroxybutyrate-co-lactate) from rice bran hydrolysate solution. These results suggest that rice bran may be a good renewable resource for the production of biomass-based polymers by recombinant microorganisms.

Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalBioresource Technology
Volume181
DOIs
StatePublished - 1 Apr 2015

Keywords

  • Escherichia coli
  • Poly(3-hydroxybutyrate)
  • Poly(3-hydroxybutyrate-co-lactate)
  • Ralstonia eutropha
  • Rice bran pretreatment

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