Rice bran treatment process for the production of 43.7 kg of hydrolysate solution containing 24.41 g/L of glucose and small amount of fructose from 5 kg of rice bran was developed and employed to produce polyhydroxyalkanoates in recombinant Escherichia coli and Ralstonia eutropha strains. Recombinant E. coli XL1-Blue expressing R. eutropha phaCAB genes and R. eutropha NCIMB11599 could produce poly(3-hydroxybutyrate) with the polymer contents of 90.1 wt% and 97.2 wt%, respectively, when they were cultured in chemically defined MR medium and chemically defined nitrogen free MR medium containing 10. mL/L of rice bran hydrolysate solution, respectively. Also, recombinant E. coli XL1-Blue and recombinant R. eutropha 437-540, both of which express the Pseudomonas sp. phaC1437 gene and the Clostridium propionicum pct540 gene could produce poly(3-hydroxybutyrate-co-lactate) from rice bran hydrolysate solution. These results suggest that rice bran may be a good renewable resource for the production of biomass-based polymers by recombinant microorganisms.
Bibliographical noteFunding Information:
This work was supported by the Technology Development Program to Solve Climate Changes (Systems Metabolic Engineering for Biorefineries) from the Ministry of Education, Science and Technology (MEST) through the National Research Foundation (NRF) of Korea (NRF-2012-C1AAA001-2012M1A2A2026556) and the Basic Science Research Program through the NRF funded by the Ministry of Education ( NRF-2013R1A1A2058379 ). Further support from the R&D Program of MOTIE/KEIT ( 10049674 ) is appreciated.
© 2015 Elsevier Ltd.
- Escherichia coli
- Ralstonia eutropha
- Rice bran pretreatment