Abstract
Rice bran treatment process for the production of 43.7 kg of hydrolysate solution containing 24.41 g/L of glucose and small amount of fructose from 5 kg of rice bran was developed and employed to produce polyhydroxyalkanoates in recombinant Escherichia coli and Ralstonia eutropha strains. Recombinant E. coli XL1-Blue expressing R. eutropha phaCAB genes and R. eutropha NCIMB11599 could produce poly(3-hydroxybutyrate) with the polymer contents of 90.1 wt% and 97.2 wt%, respectively, when they were cultured in chemically defined MR medium and chemically defined nitrogen free MR medium containing 10. mL/L of rice bran hydrolysate solution, respectively. Also, recombinant E. coli XL1-Blue and recombinant R. eutropha 437-540, both of which express the Pseudomonas sp. phaC1437 gene and the Clostridium propionicum pct540 gene could produce poly(3-hydroxybutyrate-co-lactate) from rice bran hydrolysate solution. These results suggest that rice bran may be a good renewable resource for the production of biomass-based polymers by recombinant microorganisms.
Original language | English |
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Pages (from-to) | 283-290 |
Number of pages | 8 |
Journal | Bioresource Technology |
Volume | 181 |
DOIs | |
State | Published - 1 Apr 2015 |
Bibliographical note
Publisher Copyright:© 2015 Elsevier Ltd.
Keywords
- Escherichia coli
- Poly(3-hydroxybutyrate)
- Poly(3-hydroxybutyrate-co-lactate)
- Ralstonia eutropha
- Rice bran pretreatment