Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System

Qingling Xu; Cheol Ho Heo; Gyoungmi Kim; Hyo Won Lee; Hwan Myung Kim; Juyoung Yoon

doi:10.1002/anie.201500537

Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System

Qingling Xu
, Cheol Ho Heo
, Gyoungmi Kim
, Hyo Won Lee
, Hwan Myung Kim
, Juyoung Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

240 Scopus citations

Abstract

We designed and prepared the imidazoline-2-thione containing OCl⁻ probes, PIS and NIS, which operate through specific reactions with OCl⁻ that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl⁻ generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl⁻ in live cells and tissues, indicating that this probe could have wide biological applications.

Original language	English
Pages (from-to)	4890-4894
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	16
DOIs	https://doi.org/10.1002/anie.201500537
State	Published - 13 Apr 2015

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

co-culture systems
fluorescence probes
hypochlorite generation
two-photon microscopy

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10.1002/anie.201500537

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title = "Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System",

abstract = "We designed and prepared the imidazoline-2-thione containing OCl− probes, PIS and NIS, which operate through specific reactions with OCl− that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl− generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl− in live cells and tissues, indicating that this probe could have wide biological applications.",

keywords = "co-culture systems, fluorescence probes, hypochlorite generation, two-photon microscopy",

author = "Qingling Xu and Heo, \{Cheol Ho\} and Gyoungmi Kim and Lee, \{Hyo Won\} and Kim, \{Hwan Myung\} and Juyoung Yoon",

note = "Publisher Copyright: {\textcopyright} 2015 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim",

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doi = "10.1002/anie.201500537",

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AU - Xu, Qingling

AU - Heo, Cheol Ho

AU - Kim, Gyoungmi

AU - Lee, Hyo Won

AU - Kim, Hwan Myung

AU - Yoon, Juyoung

PY - 2015/4/13

Y1 - 2015/4/13

N2 - We designed and prepared the imidazoline-2-thione containing OCl− probes, PIS and NIS, which operate through specific reactions with OCl− that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl− generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl− in live cells and tissues, indicating that this probe could have wide biological applications.

AB - We designed and prepared the imidazoline-2-thione containing OCl− probes, PIS and NIS, which operate through specific reactions with OCl− that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl− generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl− in live cells and tissues, indicating that this probe could have wide biological applications.

KW - co-culture systems

KW - fluorescence probes

KW - hypochlorite generation

KW - two-photon microscopy

UR - https://www.scopus.com/pages/publications/84926476401

U2 - 10.1002/anie.201500537

DO - 10.1002/anie.201500537

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EP - 4894

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 16

ER -

Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System

Abstract

Bibliographical note

Keywords

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