Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System

Qingling Xu, Cheol Ho Heo, Gyoungmi Kim, Hyo Won Lee, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

206 Scopus citations

Abstract

We designed and prepared the imidazoline-2-thione containing OCl probes, PIS and NIS, which operate through specific reactions with OCl that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl in live cells and tissues, indicating that this probe could have wide biological applications.

Original languageEnglish
Pages (from-to)4890-4894
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number16
DOIs
StatePublished - 13 Apr 2015

Keywords

  • co-culture systems
  • fluorescence probes
  • hypochlorite generation
  • two-photon microscopy

Fingerprint

Dive into the research topics of 'Development of Imidazoline-2-Thiones Based Two-Photon Fluorescence Probes for Imaging Hypochlorite Generation in a Co-Culture System'. Together they form a unique fingerprint.

Cite this