Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of l-lysine into cadaverine

Young Hoon Oh, Kyoung Hee Kang, Mi Jeong Kwon, Jae Woo Choi, Jeong Chan Joo, Seung Hwan Lee, Yung Hun Yang, Bong Keun Song, Il Kwon Kim, Ki Hoon Yoon, Kyungmoon Park, Si Jae Park

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A whole-cell biocatalytic system for the production of cadaverine from l-lysine has been developed. Among the investigated lysine decarboxylases from different microorganisms, Escherichia coli LdcC showed the best performance on cadaverine synthesis when E. coli XL1-Blue was used as the host strain. Six different strains of E. coli expressing E. coli LdcC were investigated and recombinant E. coli XL1-Blue, BL21(DE3) and W were chosen for further investigation since they showed higher conversion yield of lysine into cadaverine. The effects of substrate pH, substrate concentrations, buffering conditions, and biocatalyst concentrations have been investigated. Finally, recombinant E. coli XL1-Blue concentrated to an OD600 of 50, converted 192.6 g/L (1317 mM) of crude lysine solution, obtained from an actual lysine manufacturing process, to 133.7 g/L (1308 mM) of cadaverine with a molar yield of 99.90 %. The whole-cell biocatalytic system described herein is expected to be applicable to the development of industrial bionylon production process.

Original languageEnglish
Pages (from-to)1481-1491
Number of pages11
JournalJournal of Industrial Microbiology and Biotechnology
Volume42
Issue number11
DOIs
StatePublished - 1 Nov 2015

Keywords

  • Bio-catalyst
  • Cadaverine
  • Recombinant E. coli
  • Whole-cell conversion
  • l-Lysine

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