Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

Min Sang Yoo; Minguk Shin; Younghun Kim; Min Jang; Yoon E. Choi; Si Jae Park; Jonghoon Choi; Jinyoung Lee; Chulhwan Park

doi:10.1016/j.chemosphere.2017.02.060

Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

Min Sang Yoo, Minguk Shin, Younghun Kim, Min Jang, Yoon E. Choi, Si Jae Park, Jonghoon Choi, Jinyoung Lee, Chulhwan Park

Department of Chemical Engineering & Materials Science

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (10²–10¹⁰ CFU/mL) and the detection limit (10² CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.

Original language	English
Pages (from-to)	269-274
Number of pages	6
Journal	Chemosphere
Volume	175
DOIs	https://doi.org/10.1016/j.chemosphere.2017.02.060
State	Published - 2017

Bibliographical note

Funding Information:
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1A09918327) and the Basic Science Research Program through NRF funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A02036406).

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

Asian dust events
Bacillus subtilis
Biosensor
Carbon nanotubes
Pathogen

Access to Document

10.1016/j.chemosphere.2017.02.060

Cite this

@article{590f6fe9e097494e948d414a12f8aac4,

title = "Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events",

abstract = "We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (102–1010 CFU/mL) and the detection limit (102 CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.",

keywords = "Asian dust events, Bacillus subtilis, Biosensor, Carbon nanotubes, Pathogen",

author = "Yoo, {Min Sang} and Minguk Shin and Younghun Kim and Min Jang and Choi, {Yoon E.} and Park, {Si Jae} and Jonghoon Choi and Jinyoung Lee and Chulhwan Park",

note = "Funding Information: This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1A09918327) and the Basic Science Research Program through NRF funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A02036406). Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

doi = "10.1016/j.chemosphere.2017.02.060",

language = "English",

volume = "175",

pages = "269--274",

journal = "Chemosphere",

issn = "0045-6535",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

AU - Yoo, Min Sang

AU - Shin, Minguk

AU - Kim, Younghun

AU - Jang, Min

AU - Choi, Yoon E.

AU - Park, Si Jae

AU - Choi, Jonghoon

AU - Lee, Jinyoung

AU - Park, Chulhwan

N1 - Funding Information: This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1A09918327) and the Basic Science Research Program through NRF funded by the Ministry of Science, ICT & Future Planning (NRF-2015R1C1A1A02036406). Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017

Y1 - 2017

N2 - We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (102–1010 CFU/mL) and the detection limit (102 CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.

AB - We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (102–1010 CFU/mL) and the detection limit (102 CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.

KW - Asian dust events

KW - Bacillus subtilis

KW - Biosensor

KW - Carbon nanotubes

KW - Pathogen

UR - http://www.scopus.com/inward/record.url?scp=85013156171&partnerID=8YFLogxK

U2 - 10.1016/j.chemosphere.2017.02.060

DO - 10.1016/j.chemosphere.2017.02.060

M3 - Article

C2 - 28226280

AN - SCOPUS:85013156171

SN - 0045-6535

VL - 175

SP - 269

EP - 274

JO - Chemosphere

JF - Chemosphere

ER -

Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this