Development of an optimal medium for continuous ferrous iron oxidation by immobilized Acidothiobacillus ferrooxidans cells

Wan Kim Tae, Chang Joon Kim, Keun Chang Yong, Wook Ryu Hee, Kyung Suk Cho

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

This study was aimed at developing an immobilized bioreactor system in which long-term continuous ferrous iron oxidation can be realized with no formation of jarosite, which causes clogging of support pores and reactor lines. For this purpose, a medium with no jarosite formation was developed first by selecting optimal nitrogen and phosphate sources and their concentrations. Then with the developed medium containing ammonium phosphate instead of ammonium sulfate and potassium phosphate, repeated batch and continuous operations of ferrous iron oxidation by Acidothiobacillus ferrooxidans cells immobilized in a depth filter were successfully performed for an extended period of time. For about 510 h of operation including 450 h of continuous operation at dilution rates of 0.1, 0.2, and 0.3 h-1, no formation of jarosite and thus no clogging of the reactor system were observed. The maximum ferrous iron oxidation rate was as high as 2.6 g/(L·h) at a dilution rate of 0.3 h-1.

Original languageEnglish
Pages (from-to)752-759
Number of pages8
JournalBiotechnology Progress
Volume18
Issue number4
DOIs
StatePublished - 2002

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