Development of a recombinant Escherichia coli-based biocatalyst to enable high styrene epoxidation activity with high product yield on energy source

Jong Wan Bae, Eun Hee Doo, Seung Hee Shin, Sun Gu Lee, Yong Joo Jeong, Jin Byung Park, Sunghoon Park

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

A highly active recombinant whole-cell biocatalyst, Escherichia coli pETAB2/pG-KJE1, was developed for the efficient production of (S)-styrene oxide from styrene. The recombinant E. coli overexpressed styAB the genes of styrene monooxygenase of Pseudomonas putida SN1 and coexpressed the genes encoding chaperones (i.e., GroEL-GroES and DnaK-DnaJ-GrpE). The styrene monooxygenases were produced to ca. 40% of the total soluble proteins, enabling the whole-cell activity of the recombinant of 180 U/g CDW. The high StyAB activity in turn appeared to direct cofactors and molecular oxygen to styrene epoxidation. The product yield on energy source (i.e., glucose) reached ca. 40%. In addition, biotransformation in an organic/aqueous two-liquid phase system allowed the product to accumulate to 400 mM in the organic phase within 6 h, resulting in an average specific and volumetric productivity of 6.4 mmol/g dry cells/h (106 U/g dry cells) and 67 mM/h (1110 U/Laq), respectively, under mild reaction conditions. These results indicated that the high productivity and the high product yield on energy source were driven by the high enzyme activity. Therefore, it was concluded that oxygenase activity of whole-cell biocatalysts is one of the critical factors to determine their catalytic performance.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalProcess Biochemistry
Volume45
Issue number2
DOIs
StatePublished - Feb 2010

Keywords

  • (S)-styrene oxide
  • Chaperone
  • Escherichia coli
  • Styrene epoxidation
  • Styrene monooxygenase

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