Development of a human estrogen receptor dimerization assay for the estrogenic endocrine-disrupting chemicals using bioluminescence resonance energy transfer

Hye Mi Kim, Hyeyeong Seo, Yooheon Park, Hee Seok Lee, Seok Hee Lee, Kwang Suk Ko

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Endocrine-disrupting chemicals (EDCs) are found in food and various other substances, including pesticides and plastics. EDCs are easily absorbed into the body and have the ability to mimic or block hormone function. The radioligand binding assay based on the estrogen receptors binding affinity is widely used to detect estrogenic EDCs but is limited to radioactive substances and requires specific conditions. As an alternative, we developed a human cell-based dimerization assay for detecting EDC-mediated ER-alpha (ERα) dimerization using bioluminescence resonance energy transfer (BRET). The resultant novel BRET-based on the ERα dimerization assay was used to identify the binding affinity of 17β-estradiol (E2), 17α-estradiol, corticosterone, diethylhexyl phthalate, bisphenol A, and 4-nonylphenol with ERα by measuring the corresponding BRET signals. Consequently, the BRET signals from five chemicals except corticosterone showed a dose-dependent sigmoidal curve for ERα, and these chemicals were suggested as positive chemicals for ERα. In contrast, corticosterone, which induced a BRET signal comparable to that of the vehicle control, was suggested as a negative chemical for ERα. Therefore, these results were consistent with the results of the existing binding assay for ERα and suggested that a novel BRET system can provide information about EDCs-mediated dimerization to ERα.

Original languageEnglish
Article number8875
JournalInternational Journal of Environmental Research and Public Health
Volume18
Issue number16
DOIs
StatePublished - 2 Aug 2021

Keywords

  • Bioluminescence resonance energy transfer
  • Estrogen receptor
  • Estrogenic endocrine-disrupting chemical
  • Risk assessment

Fingerprint

Dive into the research topics of 'Development of a human estrogen receptor dimerization assay for the estrogenic endocrine-disrupting chemicals using bioluminescence resonance energy transfer'. Together they form a unique fingerprint.

Cite this