Determination of Serotonin Concentration in Single Human Platelets through Single-Entity Electrochemistry

Jungeun Lee, Yeji Kang, Jinho Chang, Jaewoo Song, Byung Kwon Kim

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


This research introduces a method to directly detect serotonin in a single platelet through single-entity electrochemistry. Platelets isolated from human blood were analyzed by cyclic voltammetry and current-time measurements. When a single platelet collides with an ultramicroelectrode, serotonin inside the platelet is oxidized at the electrode surface, and an anodic current peak is consequently observed during measurement. The concentration of serotonin can be determined by integrating this peak current. In addition, this method can be used to determine the platelet concentration. Analysis of the collision frequency of platelets can provide information about the platelet concentration in the blood. As a result, platelet levels and serotonin concentrations in single platelets can be measured quickly and easily.

Original languageEnglish
Pages (from-to)1943-1948
Number of pages6
JournalACS Sensors
Issue number7
StatePublished - 24 Jul 2020

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (no. 2011-0030074 and NRF-2018R1C1B6008668). The authors thank MID (Medical Illustration & Design), a part of the Medical Research Support Services of Yonsei University College of Medicine, for all artistic support related to this work.

Publisher Copyright:
Copyright © 2020 American Chemical Society.


  • human platelet detection
  • platelet serotonin
  • serotonin biosensor
  • single-entity electrochemistry
  • ultramicroelectrode


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