Determination of HCV-specific T-cell activity.

Joo Chun Yoon, Barbara Rehermann

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The magnitude and breadth of T-cell responses against HCV are associated with the outcome of HCV infection. Parameters of HCV-specific T-cell responses that are frequently assessed in clinical immunological studies include proliferation of T cells in response to HCV antigens, frequency of HCV-specific T cells secreting cytokines, and changes in antigen specificity during the course of HCV infection. Common techniques for assessing these parameters such as (3)H-thymidine incorporation assay, cytokine ELISpot assay, and strategies for epitope mapping and identification of minimal optimal epitopes are outlined, and detailed protocols are described.

Original languageEnglish
Pages (from-to)403-413
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2009


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