Determination of daumone in mouse plasma by HPLC/MS-MS

Keumhan Noh, Jong Hee Park, Minkyu Kim, Mankil Jung, Hwa Jeong Lee, Kwang Il Kwon, Wonku Kang, Hunjoo Ha

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Daumone, a pheromone secreted by Caenorhabditis elegans, is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS-MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5-week repeated oral administration of daumone in mice.

Original languageEnglish
Pages (from-to)152-155
Number of pages4
JournalBiomedical Chromatography
Issue number2
StatePublished - Feb 2012


  • Daumone
  • LC/MS/MS
  • Mouse plasma


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