Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry

Keumhan Noh; Seo Young Kim; Yoo Lim Kam; Hea Young Park Choo; Hwa Jeong Lee; Wonku Kang

doi:10.1016/j.jpba.2010.10.007

Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry

Keumhan Noh, Seo Young Kim, Yoo Lim Kam, Hea Young Park Choo, Hwa Jeong Lee, Wonku Kang

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A novel T-type calcium channel blocker, 4-amino-1-{4-[(4-chloro-phenyl)-phenyl-methyl]-piperazin-1-yl}-butan-1-one (HYP-10) has been synthesized, and the compound has shown promise as both a nociceptive and inflammatory pain reliever as well as an analgesic in a rat neuropathic pain model. A quantification method was developed for the determination of HYP-10 in rat plasma. After simple protein precipitation with methanol, HYP-10 and the internal standard, methaqualone were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma HYP-10 concentration after a single intravenous administration of the compound in rats.

Original language	English
Pages (from-to)	568-571
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	54
Issue number	3
DOIs	https://doi.org/10.1016/j.jpba.2010.10.007
State	Published - 20 Feb 2011

Bibliographical note

Funding Information:
This work was supported by grant from the Brain Korea 21 program and grant No. R15-2006-020 from the NCRC program of the MEST and the NRF through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University .

Keywords

HYP-10
LC-MS/MS
Novel T-type calcium channel blocker
Rat plasma

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title = "Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry",

abstract = "A novel T-type calcium channel blocker, 4-amino-1-{4-[(4-chloro-phenyl)-phenyl-methyl]-piperazin-1-yl}-butan-1-one (HYP-10) has been synthesized, and the compound has shown promise as both a nociceptive and inflammatory pain reliever as well as an analgesic in a rat neuropathic pain model. A quantification method was developed for the determination of HYP-10 in rat plasma. After simple protein precipitation with methanol, HYP-10 and the internal standard, methaqualone were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma HYP-10 concentration after a single intravenous administration of the compound in rats.",

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author = "Keumhan Noh and Kim, {Seo Young} and Kam, {Yoo Lim} and Choo, {Hea Young Park} and Lee, {Hwa Jeong} and Wonku Kang",

note = "Funding Information: This work was supported by grant from the Brain Korea 21 program and grant No. R15-2006-020 from the NCRC program of the MEST and the NRF through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University .",

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AU - Choo, Hea Young Park

AU - Lee, Hwa Jeong

AU - Kang, Wonku

N1 - Funding Information: This work was supported by grant from the Brain Korea 21 program and grant No. R15-2006-020 from the NCRC program of the MEST and the NRF through the Center for Cell Signaling & Drug Discovery Research at Ewha Womans University .

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AB - A novel T-type calcium channel blocker, 4-amino-1-{4-[(4-chloro-phenyl)-phenyl-methyl]-piperazin-1-yl}-butan-1-one (HYP-10) has been synthesized, and the compound has shown promise as both a nociceptive and inflammatory pain reliever as well as an analgesic in a rat neuropathic pain model. A quantification method was developed for the determination of HYP-10 in rat plasma. After simple protein precipitation with methanol, HYP-10 and the internal standard, methaqualone were chromatographed on a reversed-phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma HYP-10 concentration after a single intravenous administration of the compound in rats.

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Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography-mass spectrometry

Abstract

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Keywords

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